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Studies Found: 1335

TESTES - TESTICULAR INJURY

Inhaled hydrogen gas therapy for prevention of testicular ischemia/reperfusion injury in rats

Year Published: 2012

This study evaluated whether 2% hydrogen (H(2)) gas therapy protects against testicular ischemia/reperfusion injury which results in increased formation of reactive oxygen species and/or reactive nitrogen species, leading to testicular apoptosis and impaired spermatogenesis. Pubertal six-week-old Spraque-Dawley rats were assigned to 5 groups (10 animals/group) as follows: group A was a sham ...
LUNG - SURGERY/TRANSPLANTATION

Hydrogen-Rich Saline Attenuates Lung Ischemia-Reperfusion Injury in Rabbits

Year Published: 2012

Hydrogen gas, an antioxidant agent, was found to protect against cerebral and myocardial ischemia-reperfusion (I/R) injury. In the present study, we investigated the effect of hydrogen-rich saline (HRS) on the I/R-induced lung injury. Left lung of male New Zealand White rabbits rendered normothermic ischemia for 60 min and reperfused for up to 240 min. Treated animals received intraperitoneal ...
BRAIN - STROKE

Protective effects of hydrogen-rich saline in a rat model of permanent focal cerebral ischemia via ...

Year Published: 2012

Hydrogen gas (H(2)) as a new medical gas exerts organ-protective effects through regulating oxidative stress, inflammation and apoptosis. In contrast to H(2), hydrogen-rich saline (HS) may be more suitable for clinical application. The present study was designed to investigate whether HS can offer a neuroprotective effect in a rat model of permanent focal cerebral ischemia and what mechanism(s) ...
INTESTINE - CANCER

Effect of Reconstituted Apple Juice with Alkaline Electrolyzed Water on Cell Proliferation and ...

Year Published: 2012

Apple juice, known as a good antioxidant source, may partially protect the body from oxidative stress. In this report, the effect of apple juice reconstituted with alkaline electrolyzed water (EO-apple juice) on HT-29 cells was investigated. Specifically, the total antioxidant capacity (ORAC and TEAC) of EO-apple juice, and the effects this EO-apple juice on cell proliferation (MTT assay), DNA ...
WHOLE BODY - CANCER

Suppressive effects of electrochemically reduced water on matrix metalloproteinase-2 activities and ...

Year Published: 2012

It has been demonstrated that hydrogen peroxide (H(2)O(2)) is directly associated with elevated matrix metalloproteinase-2 (MMP-2) expression in several cell lines. Electrochemically reduced water (ERW), produced near the cathode during electrolysis, and scavenges intracellular H(2)O(2) in human fibrosarcoma HT1080 cells. RT-PCR and zymography analyses revealed that when HT1080 cells were treated ...
LIVER - NON-ALCOHOLIC STEATOHEPATITIS

Hydrogen-rich water prevents progression of nonalcoholic steatohepatitis and accompanying ...

Year Published: 2012

Oxidative stress is a strong contributor to the progression from simple fatty liver to nonalcoholic steatohepatitis (NASH). Molecular hydrogen is an effective antioxidant that reduces cytotoxic reactive oxygen species. In this study, we investigated the effects of hydrogen-rich water and the drug pioglitazone on the progression of NASH in mouse models. A methionine-choline-deficient (MCD) diet ...
WHOLE BODY - EXCERCISE

Effects of ionized alkali water intake on oxidative stress and its related gene expression induced ...

Year Published: 2012

The purpose of this study was to investigate the effects of ionized alkali water supplement on oxidative stress and proinflammatory cytokines in highly trained high school and college track athletes (n=18). In an single group and cross-over design, all subjects consumed tap water and ionized alkali water for 2 weeks in a random order, and they underwent an acute and exhaustive treadmill running at ...
KIDNEY - DIABETES (TYPE II)

Hydrogen-rich water inhibits glucose and α,β -dicarbonyl compound-induced reactive oxygen species ...

Year Published: 2012

Reactive oxygen species (ROS) production induced by α,β-dicarbonyl compounds and advanced glycation end products causes renal dysfunction in patients with type 2 diabetes and metabolic syndrome. Hydrogen-rich water (HRW) increases the H2 level in blood and tissues, thus reducing oxidative stress in animals as well as humans. In this study, we investigated the effects of HRW on glucose- and ...
BLOOD - BLOOD COMPOSITION

Colloidal platinum in hydrogen-rich water exhibits radical-scavenging activity and improves blood ...

Year Published: 2012

The 'colloidal platinum' stabilized with polyvinylpyrrolidone (Pt/PVP-colloid) was dispersed in hydrogen-rich water (HW; hydrogen concentration, 0.82 ppm; oxidation-reduction potential, -583 mV) or regular water (RW; <0.01 ppm, +218 mV). And we evaluated the antioxidant activity of Pt/PVP-colloid in HW or RW on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and improvement of blood ...
SKIN - RADIOPROTECTION

Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with ...

Year Published: 2012

Hydrogen-rich electrolyzed warm water (HW) was prepared at 41°C and exhibited dissolved hydrogen (DH) of 1.13 ppm and an oxidation-reduction potential (ORP) of -741 mV in contrast to below 0.01 ppm and+184 mV for regular warm water (RW). Fibroblasts OUMS-36 and keratinocytes HaCaT were used to examine effects of HW against UVA-ray irradiation. Type-I collagen was synthesized 1.85- to 2.03-fold ...
WHOLE BODY - CANCER

Influence of Hydrogen Discharged from Palladium Base Hydrogen Storage Alloys on Cancer Cells

Year Published: 2012

The influence of discharged hydrogen from Pd-Ni based hydrogen storage alloys (HSAs) on cultured cells has been investigated. The susceptibility of cells to discharged hydrogen varied with the kind of cells. No influence was seen in the normal cells, while an effect of killing cancer cells was observed near the HAS and the region where the cell death was observed was limited to an extent of a few ...
BRAIN - TRAUMATIC BRAIN INJURY

Protective effects of hydrogen-rich saline in a rat model of traumatic brain injury via reducing ...

Year Published: 2012

Background: Hydrogen gas (H(2)) has been considered as a novel antioxidant to selectively reduce the toxic reactive oxygen species (ROS) such as hydroxyl radical (•OH) without affecting the other signal ROS. Our recent study shows that H(2) inhalation is beneficial to traumatic brain injury (TBI) via reducing oxidative stress. In contrast to H(2), hydrogen-rich saline (HS) may be more suitable ...
TESTES - TESTICULAR INJURY

Protective Effects of Hydrogen Rich Saline Solution on Experimental Testicular Ischemia-Reperfusion ...

Year Published: 2012

We examined the effectiveness of hydrogen rich saline solution on the prevention of testicular damage induced by ischemia/reperfusion in rats. Male Sprague-Dawley® rats were divided randomly into 4 groups, including group 1-sham operated, group 2-torsion-detorsion, group 3-torsion-detorsion plus saline and group 4-torsion-detorsion plus hydrogen rich saline solution. Testicular torsion was ...
INTESTINE - SHOCK

Protective effect of hydrogen-lactated Ringer’s solution against extensive burn-induced ...

Year Published: 2012

Objective To investigate the protective effect of hydrogen-rich lactated Ringer’s solution (HRS) against intestine injury induced by extensive burn in Sprague-Dewley (SD) rat model after delayed fluid resuscitation. Methods Thirty-six male SD rats were randomly divided into three groups (n=12): sham-burn group(S group), burn plus normal lactated Ringer’s solution(NRS) group (BR group) and burn ...
BONE - RHEUMATOID ARTHRITIS

Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress ...

Year Published: 2012

Background: Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by the destruction of bone and cartilage. Although its etiology is unknown, the hydroxyl radical has been suggested to be involved in the pathogenesis of RA. Recently, molecular hydrogen (H2) was demonstrated to be a selective scavenger for the hydroxyl radical. Also, the method to prepare water containing ...
BRAIN - PARKINSON'S DISEASE

Drinking hydrogen water and intermittent hydrogen gas exposure, but not lactulose or continuous ...

Year Published: 2012

Lactulose is a synthetic disaccharide that can be catalyzed only by intestinal bacteria in humans and rodents, and a large amount of hydrogen is produced by bacterial catalysis of lactulose. We previously reported marked effects of ad libitum administration of hydrogen water on prevention of a rat model of Parkinson's disease (PD). End-alveolar breath hydrogen concentrations were measured in 28 ...
BRAIN - BRAIN INJURY

Beneficial effect of hydrogen-rich saline on cerebral vasospasm after experimental subarachnoid ...

Year Published: 2012

Cerebral vasospasm (CV) remains a common and devastating complication in patients with subarachnoid hemorrhage (SAH). Despite its clinical significance and extensive research, the underlying pathogenesis and therapeutic perspectives of CV remain incompletely understood. Recently, it has been suggested that molecular hydrogen (H(2)) can selectively reduce levels of hydroxyl radicals (·OH) and ...
BRAIN - TRAUMATIC BRAIN INJURY

Hydrogen-rich saline protects against oxidative damage and cognitive deficits after mild traumatic ...

Year Published: 2012

Oxidative stress is the principal factor in traumatic brain injury (TBI) that initiates events that result in protracted neuronal dysfunction and remodeling. Importantly, antioxidants can protect the brain against oxidative damage and modulate the capacity of the brain to cope with synaptic dysfunction and cognitive impairment. However, no studies have investigated the effects of hydrogen-rich ...
SKIN - RADIOPROTECTION

Hydrogen-rich saline protects against ultraviolet B radiation injury in rats

Year Published: 2012

Exposure of skin to solar ultraviolet (UV) radiation induces photo-damage. Ultraviolet B (UVB) is the major component of UV radiation which induces the production of reactive oxygen species (ROS) and plays an important role in photo-damage. Hydrogen gas reduces ROS and alleviates inflammation. In this study, we sought to demonstrate that hydrogen-rich saline has the effect on skin injuries caused ...
HEART - CARDIAC ARREST

H2 gas improves functional outcome after cardiac arrest to an extent comparable to therapeutic ...

Year Published: 2012

All clinical and biological manifestations related to postcardiac arrest (CA) syndrome are attributed to ischemia-reperfusion injury in various organs including brain and heart. Molecular hydrogen (H(2)) has potential as a novel antioxidant. This study tested the hypothesis that inhalation of H(2) gas starting at the beginning of cardiopulmonary resuscitation (CPR) could improve the outcome of CA. ...
SKIN - WOUND HEALING

Feasibility study of the sterilization of pigskin used as wound dressings by neutral electrolyzed ...

Year Published: 2012

Background: Neutral electrolyzed water (NEW) is considered to be a high-level biodegradable disinfectant with sporicidal, bactericidal, and virucidal activity. It has also been reported to accelerate wound healing; thus, it is particularly attractive for the elimination or minimization of the microbial population of skin grafts to be used as wound dressings. Methods: Pigskins were sterilized with ...
BRAIN - BRAIN INJURY

Inhalation of hydrogen gas attenuates cognitive impairment in transient cerebral ischemia via ...

Year Published: 2012

To investigate the effects of inhalation of hydrogen gas on cognitive impairment induced by transient cerebral ischemia and its potential mechanism. Two-vessel occlusion rat model was used to produce 10-minute transient global cerebral ischemia. One hundred and twenty male Wistar rats were randomly divided into sham, sham+H(2), ischemia, and ischemia+H(2) groups (n = 30 each group). Inhalation of ...
EYE - RADIOPROTECTION

Protective effect of saturated hydrogen saline against blue light-induced retinal damage in rats

Year Published: 2012

To explore the effect of saturated hydrogen saline on blue light-induced retinal damage in rats. The retinal damage of rats was induced by blue light exposure for 6 hours and examined 8 hours, 16 hours and 24 hours after the exposure. One hundred female Sprague-Dawley rats were randomly divided into four groups. Group 1 included 30 rats received light exposure without any other treatment. Group 2 ...
WHOLE BODY - SURGERY/TRANSPLANTATION

Insufflation of Hydrogen Gas Restrains the Inflammatory Response of Cardiopulmonary Bypass in a Rat ...

Year Published: 2012

Systemic inflammatory responses in patients receiving cardiac surgery with the use of the cardiopulmonary bypass (CPB) significantly contribute to CPB-associated morbidity and mortality. We hypothesized that insufflated hydrogen gas (H(2) ) would provide systemic anti-inflammatory and anti-apoptotic effects during CPB, therefore reducing proinflammatory cytokine levels. In this study, we examined ...
TESTES - RADIOPROTECTION

Hydrogen-rich saline protects spermatogenesis and hematopoiesis in irradiated BALB/c mice

Year Published: 2012

Background: Recent studies show that molecular hydrogen (dihydrogen, H2) has potential as an effective and safe radioprotective agent through reducing oxidative stress. The aim of this study was to investigate whether H2 is able to protect spermatogenesis and hematopoiesis from radiation-induced injuries. Material/methods: H2 was dissolved in physiological saline using an apparatus produced by our ...