Protective Effects of Different Kinds of Filtered Water on Hypertensive Mouse by Suppressing Oxidative Stress and Inflammation

Oxidative stress and inflammation play an important role in hypertensive animals and patients. Hydrogen plays a role of antioxidation and anti-inflammation. Calcium and magnesium play an important role in reducing hypertension and antioxidant. Filtered water contains abundant hydrogen and a large number of other essential elements of the human body. We investigated the protective effects of filtered water on hypertensive mice. To establish hypertension model, ICR mice were administered with N-nitro-L-arginine methyl ester (L-NAME) hydrochloride 64 mg/kg per day for 1 month. The hypertensive mice were, respectively, administered with pure water, tap water, and filtered water for 2 months. Lipid peroxidation, antioxidant enzymatic activity, endothelin-1 (ET-1), angiotensin II (Ang II), and proinflammatory cytokines (TNF-α, IL-6, and IL-1β) were assessed. Expressions of phosphorylated NF-κB P65 in the kidney were analyzed by western blot. qRT-PCR analysis was adopted to determine the expression levels of the proinflammatory cytokines and NF-κB P65. The results demonstrated that filtered water can reduce the blood pressure. Filtered water treatment restored the activity of antioxidant enzymes, downregulated ET-1, and Ang II in the serum of mice. Filtered water treatment suppressed proinflammatory cytokines and decreased the mRNA expression of TNF-α, IL-6, IL-1β, and NF-κB P65. Consumption of filtered water inhibited the expression of NF-κB P65. This suggests that filtered water can reduce the blood pressure. The protection mechanisms include downregulating oxidative stress and inhibiting inflammation, which is partly due to the inhibition of the NF-κB signaling pathway.

UPLC/MS-Based Metabolomics Investigation of the Protective Effect of Hydrogen Gas Inhalation on Mice with Calcium Oxalate-Induced Renal Injury

Hydrogen has a significant protective effect on calcium oxalate-induced renal injury, but its effect on metabolic profiles is unknown. This study showed the effects of hydrogen on serum and urine metabolites in a renal injury model. Ultra-HPLC quadrupole time-of-flight-MS-based metabolomics was used to characterise metabolic variations. Twenty-five serum metabolites and 14 urine metabolites showed differences in the the nitrogen and oxygen inhalation (NO), nitrogen and oxygen inhalation combined with calcium oxalate induction (CaOx), and hydrogen inhalation combined with calcium oxalate induction (HO+CaOx) groups. Nineteen serum metabolites and 7 urine metabolites showed significant restoration to normal levels after hydrogen gas (H2) treatment. These metabolites are primarily related to amino acid metabolism, fatty acid metabolism, and phospholipid metabolism. This study showed that a comprehensive metabolomics approach is an effective strategy to elucidate the mechanisms underlying the effects of hydrogen treatment on calcium oxalate-induced renal injury.

Itraq-Based Quantitative Proteomic Analysis of Lungs in Murine Polymicrobial Sepsis with Hydrogen Gas Treatment

Sepsis-associated acute lung injury (ALI), which carries a high morbidity and mortality in patients, has no effective therapeutic strategies to date. Our group has already reported that hydrogen gas (H2) exerts a protective effect against sepsis in mice. However, the molecular mechanisms underlying H2 treatment are not fully understood. This study investigated the effects of H2 on lung injuries in septic mice through the isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analysis. Male ICR mice used in this study were subjected to cecal ligation and puncture (CLP) or sham operation. And 2% H2 was inhaled for 1 h beginning at 1 and 6 h after sham or CLP operation. The iTRAQ-based liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was preformed to investigate lung proteomics. Sepsis-challenged animals had decreased survival rate, as well as had increased bacterial burden in blood, peritoneal lavage, and lung sample, which were significantly ameliorated by H2 treatment. Moreover, a total of 4,472 proteins were quantified, and 192 differentially expressed proteins were related to the protective mechanism of H2 against sepsis. Functional enrichment analysis showed that H2-related differential proteins could be related to muscle contraction, oxygen transport, protein synthesis, collagen barrier membranes, cell adhesion, and coagulation function. These proteins were significantly enriched in four signaling pathways, and two of which are associated with coagulation. In addition, H2 alleviates ALI in septic mice through downregulating the expression of Sema 7A, OTULIN, and MAP3K1 as well as upregulating the expression of Transferrin. Thus, our findings provide an insight into the mechanism of H2 treatment in sepsis by proteomic approach, which may be helpful to the clinic application of H2 in patients with sepsis.

Abstract 556: Protective Effect of a Hydrogen-Rich Preservation Solution During Cold Ischemia in Rat Lung Transplantation

No abstract available

Effects of hydrogen-rich water on attenuating fatigue induced by daily activities or mental tasks

Objectives In 2007, the first report that hydrogen removes active oxygen was published, and many clinical and non-clinical studies have since been conducted using hydrogen gas or water. Several diseases and symptoms are related to active oxygen, and fatigue is also induced by increases in oxidative stress. The current randomized, double-blind, placebo-controlled, crossover study was conducted to investigate the effects of hydrogen-rich water(HRW)on attenuating fatigue induced by daily activities or mental tasks. Methods Twenty-four healthy volunteers were randomized to either oral administration of hydrogen-rich water containing 0.36 mg of hydrogen or placebo for 4 weeks. Subjective sensation and work efficacy were examined using the visual analogue scale(VAS)and the advanced trail making test(ATMT), respectively, to evaluate the anti-fatigue effects of hydrogen-rich water. In addition, we measured serum malondialdehyde-modified low density lipoprotein (MDA-LDL)and derivatives of reactive oxygen metabolites(d-ROMs)as possible mechanisms of action. Results On the VAS, the hydrogen-rich water group had a significantly lower sleepiness and tension score after 4-week administration, and a significantly higher motivation and relaxation score after mental tasks compared with the placebo group. On the ATMT, hydrogen-rich water significantly reduced the mean reaction time during mental tasks. The serum MDA-LDL level after 4-week administration was significantly lower compared with before administration in the hydrogen-rich water group. The serum d-ROM level was significantly correlated with the VAS fatigue sensation score in the hydrogen-rich water group. Conclusions These results suggest that hydrogen-rich water improves several subjective sensations accompanying fatigue induced by daily activities and mental tasks by enhancing work efficacy and attenuating fatigue during mental tasks. We consider these effects to be caused by the antioxidant action of hydrogen.

Local generation of hydrogen for enhanced photothermal therapy

By delivering the concept of clean hydrogen energy and green catalysis to the biomedical field, engineering of hydrogen-generating nanomaterials for treatment of major diseases holds great promise. Leveraging virtue of versatile abilities of Pd hydride nanomaterials in high/stable hydrogen storage, self-catalytic hydrogenation, near-infrared (NIR) light absorption and photothermal conversion, here we utilize the cubic PdH0.2 nanocrystals for tumour-targeted and photoacoustic imaging (PAI)-guided hydrogenothermal therapy of cancer. The synthesized PdH0.2 nanocrystals have exhibited high intratumoural accumulation capability, clear NIR-controlled hydrogen release behaviours, NIR-enhanced self-catalysis bio-reductivity, high NIR-photothermal effect and PAI performance. With these unique properties of PdH0.2 nanocrystals, synergetic hydrogenothermal therapy with limited systematic toxicity has been achieved by tumour-targeted delivery and PAI-guided NIR-controlled release of bio-reductive hydrogen as well as generation of heat. This hydrogenothermal approach has presented a cancer-selective strategy for synergistic cancer treatment.

In vivo quantification of hydrogen gas concentration in bone marrow surrounding magnesium fracture fixation hardware using an electrochemical hydrogen gas sensor

Statement of significance: An electrochemical H2 sensor was used to monitor the degradation of a Mg fracture fixation system in a lapine ulna fracture model. Interestingly, the H2 concentration in the bone marrow is 82% higher than H2 saturated water solution. This suggests H2 generated in situ is trapped in the bone marrow and bone is less permeable than the surrounding tissues. The detectable H2 at the rabbit skin also demonstrates a H2 sensor’s ability to monitor the degradation process under thin layers of tissue. H2 sensing shows promise as a tool for monitoring the degradation of Mg alloy in vivo and creating in vitro test beds to more mechanistically evaluate the effects of varying H2 concentrations on cell types relevant to osteogenesis.

Magnesium Particles Coated with Mesoporous Nanoshells as Sustainable Therapeutic-Hydrogen Suppliers to Scavenge Continuously Generated Hydroxyl Radicals in Long Term

Sustainable supplementation of massive molecular‐H2 is considered to be the most effective therapy for long‐term elimination of excessive hydroxyl radicals (·OH) in vivo, but has not been achieved so far. In this work, it is demonstrated that magnesium microparticles (Mg MPs) coated with mesoporous nanoshells can achieve the long‐term and high‐efficient generation of therapeutic hydrogen in physiological condition for ·OH scavenging. The as‐proposed magnesium@mesoporous SiO2 core–shell microparticles (Mg@p‐SiO2 MPs) are synthesized by developing a modified Stöber method using acetone as the solvent, and they exhibit shell thickness (d)‐dependent H2 release behavior due to the barrier effect of nanoshells on both the occurrence of Mg–water reaction and H2 diffusion. Consequently, they are able to provide vast quantities of H2 molecules dissolved in body fluid with a rate controlled by d over a long period. A simulation model is established which well explains and further predicts the dependence of H2 release behavior on d, and the long‐term protection of cells from oxidative damage by Mg@p‐SiO2 MPs is also experimentally validated. As the H2 concentration and effective duration in medium can be adjusted by dosage and d, Mg@p‐SiO2 MPs are promising for accurate H2 drug delivery in vivo. The magnesium@mesoporous SiO2 core–shell microparticles (Mg@p‐SiO2 MPs) can achieve the long‐term and high‐efficient generation of therapeutic hydrogen in physiological condition for ·OH scavenging, which are synthesized by developing a modified Stöber method using acetone as the solvent and protect cells from oxidative damage for a long time.

Abstract 225: 28-Days Hydrogen-Rich Water Supplementation Affects Exercise Capacity in Mid-Age Overweight Women

No abstract available

Positive effects of hydrogen-water bathing in patients of psoriasis and parapsoriasis en plaques

Psoriasis and parapsoriasis en plaques are chronic inflammatory skin diseases, both representing therapeutic challenge in daily practice and adversely affecting the quality of life. Reactive oxygen species (ROS) has been evidenced to be involved in the pathogenesis of the chronic inflammatory diseases. We now report that hydrogen water, an effective ROS scavenger, has significant and rapid improvement in disease severity and quality of life for patients with psoriasis and parapsoriasis en plaques. At week 8, our parallel-controlled trial revealed 24.4% of patients (10/41) receiving hydrogen-water bathing achieved at least 75% improvement in Psoriasis Area Severity Index (PASI) score compared with 2.9% of patients (1/34) of the control group (Pc = 0.022, OR = 0.094, 95%CI = [0.011, 0.777]). Of patients, 56.1% (23/41) who received bathing achieved at least 50% improvement in PASI score compared with only 17.7%(6/34) of the control group (P = 0.001, OR = 0.168, 95%CI = [0.057, 0.492]). The significant improvement of pruritus was also observed (P = 3.94 × 10-4). Besides, complete response was observed in 33.3% of patients (2/6) of parapsoriasis en plaques and partial response in 66.7% (4/6) at week 8. Our findings suggested that hydrogen-water bathing therapy could fulfill the unmet need for these chronic inflammatory skin diseases.