Molecular Hydrogen Suppresses Renal Injury in Chronic Kidney Disease Rats

Background: Cathode side commercial hydrogen dissolved water (HW) exhibits low dissolved oxygen, high dissolved hydrogen and significant negative redox potential. Therapeutic applications of HW have recently been reported. Thus, the present study aimed to examine effects of HW consumption on renal injury in a rat model of chronic kidney disease.Methods: Twenty Dahl S rats were given HW and tap water (TW), over a 4-week period. Thereafter, they were allocated to either group; non stressed (NS), and oxi-carbonyl stressed (OC: 5% salt diet and 1% methylglyoxal in drinking water) group, respectively (HW, TW n=5 each group). OS groups were subjected to unilateral kidney ischemic reperfusion (IR) in the final week.Results: No differences were found in blood pressure and urinary parameters between HW and TW. Glomerular adhesion rates in the IR kidney and positive osteopontin of the non-IR kidney were significantly higher in OC rats on HW, respectively. ED-1 staining on HW was significantly lower than TW in both the IR and non-IR kidneys of the OC rats. Plasma MCP-1 was significantly lower on HW after IR.Conclusion: Drinking HW at least partly suppressed renal damage in rats with combination of oxidative, carbonyl and ischemic stimulus.