Radio-protective effect of hydrogen rich water combined with amifostine in mice

Background: Hydrogen has been demonstrated can selectively reduce the hydroxyl, which is the main cause of ionizing radiation-induced damage. Amifostine (AM) is the only radioprotective drug approved by the U.S. Food and Drug Administration for use in radiotherapy. The purpose of the present study was to investigate the combined radio-protective effect of hydrogen rich water (HRW) and AM. Materials and Methods: Male ICR mice were treated intragastrically with HRW or/and intraperitoneally with AM 30 minutes prior to 9.0 Gy whole body irradiation from a⁶⁰Co source (dose rate 0.96Gy/min). Then the survival rate for 30 days, the hematological parameters, the Clinical chemistry parameters and the bone marrow nucleated cells were examined. Results: We found that the mice treated with HRW and AM before irradiaion could increase the 30-day survival rate and improve the body weight better than the HRW or AM treatment alone group and irradiaion alone group. Hematological test and Clinical chemistry assays also showed the same results that HRW combined AM could beter improve the recovery of hemopoietic system and alleviate the detrimental effects of radiation. Conclusion: The results indicate that the combined application of HRW and AM may be a beter method for radiaion therapy. © 2016, Novim Medical Radiation Institute. All rights reserved.

Changes in IL-4 and IL-13 expression in allergic-rhinitis treated with hydrogen-rich saline in guinea-pig model

Background: Medical gas hydrogen (H2) has a special role in airway inflammation; however, the effect of H2 on allergic rhinitis (AR) remains unclear. This study explored the possible roles of H2 on the pathogenesis of AR and observed the influences of H2 on cytokines IL-4 and IL-13. Methods: An AR guinea pig model was established by nasal ovalbumin sensitisation. Eighteen guinea pigs were divided into three groups, namely, saline control, AR-sensitised, and hydrogen-rich saline (HRS)-treated groups, with each group having six guinea pigs. The frequencies of sneezing and scratching were recorded. The IgE level and cytokine (IL-4 and IL-13) levels in the serum were measured. The expression levels of IL-4 and IL-13 mRNA and protein in the nasal mucosa were also determined by real-time reverse transcriptase-polymerase chain reaction and Western blot. We also observed the infiltration of cytokine (IL-4 and IL-13) in nasal mucosa by immunofluorescence. Results: The frequencies of sneezing and scratching, as well as the levels of IgE, IL-4, and IL-13, in the serum were higher in the AR group than in the control group (p<0.01), whereas all these parameters were decreased significantly after HRS treatment (p<0.05). The expression levels of IL-4 and IL-13 mRNA and protein in the nasal mucosa were also lower in guinea pigs treated with HRS than those in the AR group (p<0.05). Conclusions: HRS could affect anti-inflammation in AR and decreased the expression of IL-4 and IL-13.