Antibacterial Activity of Hydrogen-rich Water Against Oral Bacteria

There are estimated to be about 700 species of bacteria inthe oral cavity. Based on epidemiological investigations,some of these strains have been proposed as the pathogensresponsible for oral diseases such as dental caries, gingivitisand periodontitis. Since electrolyzed hydrogen-rich waterhas been shown to have beneficial effects on human immunity,its use has increased. In our study, the antibacterialactivity of hydrogen-rich water for oralagainst bacteria associatedwith oral disease was evaluated. The bacterial strainsStreptococcus mutans, Fusobacterium nucleatum, Porphyromonasgingivalis and Tannerella forsythia were culturedin specific growth medium. S. mutans, F. nucleatum and P.gingivalis were soaked to thein both hydrogen water andtap water for 30 sec and then inoculated onto mitis-salivariusagar and brain heart infusion agar including supplementedwithvitamin K and hemin, respectively. The numbersof bacterial colonies were then measured after cultivationfor 48 hours. In the case of T. forsythia, which does notgrow well on agar plates, inoculations into modified neworal spirochete (NOS) broth were performed and growth curveanalysis was undertaken every day with a spectrophotometer.Hydrogen water showed antibacterial activity against allfour bacterial strains in comparison with tap-water. We conclude from this that hydrogen water may have a positiveimpact on oral hygiene by helping to remove cariogenicbacteria and periodontopathogens.

Antifungal effect of electrolyzed hydrogen water on Candida albicans biofilm

Purpose: Candida albicans can cause mucosal disease in many vulnerable patients. Also they are associated with denture-related stomatitis. Electrolyzed water is generated by electric current passed via water using various metal electrodes and has antimicrobial activity. The aim of this study was to investigate antifungal activity of electrolyzed water on C. albicans biofilm. Materials and Methods: C. albicans was cultured by sabouraud dextrose broth and F-12 nutrient medium in aerobic and 5% CO2 condition to form blastoconidia (yeast) and hyphae type, respectively. For formation of C. albicans biofilm, C. albicans was cultivated on rough surface 6-well plate by using F-12 nutrient medium in CO2 incubator for 48 hr. After electrolyzing tap water using various metal electrodes, the blastoconidia and hyphal type of C. albicans were treated with electrolyzed water. C. albicans formed blastoconidia and hyphae type when they were cultured by sabouraud dextrose broth and F-12 nutrient medium, respectively. Results: The electrolyzed water using palladium electrode (EWP) exhibited antifungal effect on blastoconidia of C. albicans. Also, the EWP significantly has antifungal activity against C. albicans biofilm and hyphae. In the electrolyzed water using various metal electrodes, only the EWP have antifungal activity. Conclusion: The EWP may use a gargle solution and a soaking solution for prevention of oral candidiasis and denture-related stomatitis due to antifungal activity.