The effect of donor treatment with hydrogen on lung allograft function in rats

Atsunori Nakao, Chien-Sheng Huang, Kosuke Masutani, Meinoshin Okumura, Norihisa Shigemura, Timothy R. Billiar, Tomohiro Kawamura, Ximei Peng, Yoshiya Toyoda

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DOI: 10.1016/j.surg.2011.05.019 DOI is the universal ID for this study.

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Because inhaled hydrogen provides potent anti-inflammatory and antiapoptotic effects against acute lung injury, we hypothesized that treatment of organ donors with inhaled hydrogen during mechanical ventilation would decrease graft injury after lung transplantation. Orthotopic left lung transplants were performed using a fully allogeneic Lewis to Brown Norway rat model. The donors were exposed to mechanical ventilation with 98% oxygen plus 2% nitrogen or 2% hydrogen for 3 h prior to harvest, and the lung grafts underwent 4 h of cold storage in Perfadex (Vitrolife, Göteborg, Sweden). The graft function, histomorphologic changes, and inflammatory reactions were assessed. The combination of mechanical ventilation and prolonged cold ischemia resulted in marked deterioration of gas exchange when the donors were ventilated with 2% nitrogen/98% oxygen, which was accompanied by upregulation of proinflammatory cytokines and proapoptotic molecules. These lung injuries were attenuated significantly by ventilation with 2% hydrogen. Inhaled hydrogen induced heme oxygenase-1, an antioxidant enzyme, in the lung grafts prior to implantation, which might contribute to protective effects afforded by hydrogen. Preloaded hydrogen gas during ventilation prior to organ procurement protected lung grafts effectively from ischemia/reperfusion-induced injury in a rat lung transplantation model.

Publish Year 2011
Country United States
Rank Positive
Journal Surgery
Primary Topic Lung
Secondary TopicSurgery/Transplantation
Model Rat
Tertiary TopicTransplantation/Graft Injury
Vehicle Gas
pH N/A
Application Ventilation