Protective effects of a hydrogen-rich solution during cold ischemia in rat lung transplantation

Hidenao Kayawake, Hiroshi Date, Mamoru Takahashi, Masao Saito, Ryosuke Kurokawa, Shin-Ichi Hirano, Toyofumi F. Chen-Yoshikawa, Yuhei Yokoyama

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DOI: 10.1016/j.jtcvs.2019.09.175 DOI is the universal ID for this study.

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Background: Molecular hydrogen can reduce the oxidative stress of ischemia-reperfusion injury in various organs for transplantation and potentially improve survival rates in recipients. This study aimed to evaluate the protective effects of a hydrogen-rich preservation solution against ischemia-reperfusion injury after cold ischemia in rat lung transplantation.

Methods: Lewis rats were divided into a nontransplant group (n = 3), minimum-ischemia group (n = 3), cold ischemia group (n = 6), and cold ischemia with hydrogen-rich (more than 1.0 ppm) preservation solution group (n = 6). The rats in the nontransplant group underwent simple thoracotomy, and the rats in the remaining 3 groups underwent orthotopic left lung transplantation. The ischemic time was <30 minutes in the minimum-ischemia group and 6 hours in the cold ischemia groups. After 2-hour reperfusion, we evaluated arterial blood gas levels, pulmonary function, lung wet-to-dry weight ratio, and histologic features of the lung tissue. The expression of proinflammatory cytokines was measured using quantitative polymerase chain reaction assays, and 8-hydroxydeoxyguanosine levels were evaluated using enzyme-linked immunosorbent assays.

Results: When compared with the nontransplant and minimum-ischemia groups, the cold ischemia group had lower dynamic compliance, lower oxygenation levels, and higher wet-to-dry weight ratios. However, these variables were significantly improved in the cold ischemia with hydrogen-rich preservation solution group. This group also had fewer signs of perivascular edema, lower interleukin-1β messenger RNA expression, and lower 8-hydroxydeoxyguanosine levels than the cold ischemia group. Conclusions: The use of a hydrogen-rich preservation solution attenuates ischemia-reperfusion injury in rat lungs during cold ischemia through antioxidant and anti-inflammatory effects.

Publish Year 2019
Country Japan
Rank Positive
Journal Journal of Thoracic and Cardiovascular Surgery
Primary Topic Lung
Secondary TopicSurgery/Transplantation
Model Rat
Tertiary TopicTransplantation/Graft Injury
Vehicle Solution (Dissolved)
pH Neutral
Application Immersion