Molecular hydrogen attenuates hypoxia/reoxygenation injury of intrahepatic cholangiocytes by activating Nrf2 expression

Baochun Lu, Guixing Jiang, Haijun Tang, Jianhua Yu, Peitu Ren, Rongguo Zhang, Weiguang Zhang, Xinxian Ruan

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DOI: 10.1016/j.toxlet.2015.08.010 DOI is the universal ID for this study.

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Hypoxia/reoxygenation (H/R) injury of cholangiocytes causes serious biliary complications during hepatobiliary surgeries. Molecular hydrogen (H2) has been shown to be effective in protecting various cells and organs against oxidative stress injury. Human liver cholangiocytes were used to determine the potential protective effects of hydrogen against cholangiocyte H/R injury and explore the underlying mechanisms. We found that H2 ameliorated H/R-induced cholangiocytes apoptosis. Our study revealed that H2 activated NF-E2-related factor 2 (Nrf2) and downstream cytoprotective protein expression. However, the protective function of H2 was abolished when Nrf2 was silenced. Apoptosis in cholangiocytes isolated from a rat model of liver ischemia/reperfusion injury indicated that H2 significantly attenuates ischemia/reperfusion cholangiocyte injury in vivo. In conclusion, our study shows that H2 protects intrahepatic cholangiocytes from hypoxia/reoxygenation-induced apoptosis in vitro or in vivo, and this phenomenon may depend on activating Nrf2 expression.

Publish Year 2015
Country China
Rank Positive
Journal Toxicology Letters
Primary Topic Liver
Secondary TopicSurgery/Transplantation
Model Cell Culture
Tertiary TopicIschemia-Reperfusion Injury
Vehicle Medium (Dissolved)
pH Neutral
Application Culture Media