Molecular hydrogen attenuates hypoxia/reoxygenation injury of intrahepatic cholangiocytes by activating Nrf2 expression
Baochun Lu, Guixing Jiang, Haijun Tang, Jianhua Yu, Peitu Ren, Rongguo Zhang, Weiguang Zhang, Xinxian Ruan
Abstract:
Hypoxia/reoxygenation (H/R) injury of cholangiocytes causes serious biliary complications during hepatobiliary surgeries. Molecular hydrogen (H2) has been shown to be effective in protecting various cells and organs against oxidative stress injury. Human liver cholangiocytes were used to determine the potential protective effects of hydrogen against cholangiocyte H/R injury and explore the underlying mechanisms. We found that H2 ameliorated H/R-induced cholangiocytes apoptosis. Our study revealed that H2 activated NF-E2-related factor 2 (Nrf2) and downstream cytoprotective protein expression. However, the protective function of H2 was abolished when Nrf2 was silenced. Apoptosis in cholangiocytes isolated from a rat model of liver ischemia/reperfusion injury indicated that H2 significantly attenuates ischemia/reperfusion cholangiocyte injury in vivo. In conclusion, our study shows that H2 protects intrahepatic cholangiocytes from hypoxia/reoxygenation-induced apoptosis in vitro or in vivo, and this phenomenon may depend on activating Nrf2 expression.
| Publish Year |
2015 |
| Country |
China |
| Rank |
Positive |
| Journal |
Toxicology Letters |
| Primary Topic |
Liver |
| Secondary Topic | Surgery/Transplantation |
| Model |
Cell Culture |
| Tertiary Topic | Ischemia-Reperfusion Injury |
| Vehicle |
Medium (Dissolved) |
| pH |
Neutral |
| Application |
Culture Media |
| Comparison |
|
| Complement |
|
Hydrogen gas alleviates acute ethanol-induced hepatotoxicity in mice via modulating TLR4/9 innate ...
2023 - Liver - Fatty Liver Disease (Alcoholic)
Alcoholic liver disease (ALD), which is induced by chronic heavy alcohol consumption, accompanies complicated pathological mechanisms, including oxidative stress, inflammation, cell death, epigenetic changes and acetaldehyde-mediated toxicity. ...
Investigating the Effect of Hydrogen-Rich Water on Liver Cell Injury and Liver Cancer by Regulating ...
Background Liver cancer is an extremely heterogeneous malignant disease among tumors identified to date. In recent years, a large number of studies have found that low-concentration hydrogen or hydrogen-rich water or hydrogen-saturated physiological ...
The Effect of Adjuvant Therapy with Molecular Hydrogen on Endogenous Coenzyme Q10 Levels and ...
2023 - Liver - Fatty Liver Disease (Nonalcoholic)
Molecular hydrogen (H2) has been recognized as a novel medical gas with antioxidant and anti-inflammatory effects. Non-alcoholic fatty liver disease (NAFLD) is a liver pathology with increased fat accumulation in liver tissue caused by factors other ...
Hydrogen attenuates endothelial glycocalyx damage associated with partial cardiopulmonary bypass in ...
2023 - Endothelium - Surgery/Transplantation
Cardiopulmonary bypass (CPB) causes systemic inflammation and endothelial glycocalyx damage. Hydrogen has anti-oxidant and anti-inflammatory properties; therefore, we hypothesized that hydrogen would alleviate endothelial glycocalyx damage caused by ...
Hydrogen Attenuates Inflammation by Inducing Early M2 Macrophage Polarization in Skin Wound Healing
2023 - Skin - Surgery/Transplantation
The heterogeneous and highly plastic cell populations of macrophages are important mediators of cellular responses during all stages of wound healing, especially in the inflammatory stage. Molecular hydrogen (H2), which has potent antioxidant and ...
Hydrogen-generating Si-based agent improves fat graft survival in rats
2023 - Skin - Surgery/Transplantation
Background: Regulating excessive inflammation and oxidative stress in fat grafting may improve retention rates. Hydrogen effectively combats oxidative stress and inflammation and reportedly inhibits ischemia-reperfusion injury in various organs. ...