Influence of Hydrogen-rich Saline on Hepatocyte Autophagy During Laparoscopic Liver Ischaemia-reperfusion Combined Resection Injury in Miniature Pigs
Ge Bai, Hong-Bin Wang, Hui Li, Jiantao Zhang, Mingzi Chen, Qianzhen Zhang, Tao Liu, Yansong Ge
Abstract:
Introduction: The purpose of this study was to investigate the protective effect of hydrogen-rich saline (HRS) against liver ischaemia-reperfusion combined resection injury. Material and
Methods: Eighteen miniature pigs were randomly divided into three groups: a sham operated group (sham group, laparoscopic liver ischaemia-reperfusion combined resection injury group (IRI group), and a hydrogen-rich saline intervention group (IRI + HRS group). Samples of hepatic tissue and serum were collected at the time of reperfusion and then 3 h, 1 d, and 3 d post reperfusion. Liver function, oxidative stress, autophagy-related mRNA genes, and protein expression were evaluated. Changes in cell and tissue ultrastructure were examined by transmission electron microscopy.
Results: Compared with the sham group, the level of autophagy of hepatocytes increased in the IRI and IRI + HRS groups, corresponding to high oxidative stress and severe liver function injury. Liver function, antioxidant content, autophagy levels, and liver injury were improved after intervention with HRS in the IRI + HRS group compared with the IRI group.
Conclusion: Intervention with hydrogen-rich saline could exert a protective effect against liver ischaemia-reperfusion combined resection injury through the reduction of oxidative stress and hepatocyte autophagy.
| Publish Year |
2018 |
| Country |
China |
| Rank |
Positive |
| Journal |
Journal of Veterinary Research |
| Primary Topic |
Liver |
| Secondary Topic | Surgery/Transplantation |
| Model |
Pig |
| Tertiary Topic | Ischemia-Reperfusion Injury |
| Vehicle |
Saline (Dissolved) |
| pH |
Neutral |
| Application |
Injection |
| Comparison |
|
| Complement |
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