Hydrogen supplementation of preservation solution improves viability of osteochondral grafts

Bunpei Sato, Gen Inoue, Jun Kuzuno, Kenji Onuma, Kentaro Uchida, Masanobu Ujihira, Masashi Takaso, Rina Sakai, Ryosuke Kurokawa, Takuya Yamada

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DOI: 10.1155/2014/109876 DOI is the universal ID for this study.

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Allogenic osteochondral tissue (OCT) is used for the treatment of large cartilage defects. Typically, OCTs collected during the disease-screening period are preserved at 4°C; however, the gradual reduction in cell viability during cold preservation adversely affects transplantation outcomes. Therefore, improved storage methods that maintain the cell viability of OCTs are needed to increase the availability of high-quality OCTs and improve treatment outcomes. Here, we evaluated whether long-term hydrogen delivery to preservation solution improved the viability of rat OCTs during cold preservation. Hydrogen-supplemented Dulbecco's Modified Eagles Medium (DMEM) and University of Wisconsin (UW) solution both significantly improved the cell viability of OCTs during preservation at 4°C for 21 days compared to nonsupplemented media. However, the long-term cold preservation of OCTs in DMEM containing hydrogen was associated with the most optimal maintenance of chondrocytes with respect to viability and morphology. Our findings demonstrate that OCTs preserved in DMEM supplemented with hydrogen are a promising material for the repair of large cartilage defects in the clinical setting.

Publish Year 2014
Country Japan
Rank Positive
Journal Scientific World Journal
Primary Topic Bone
Secondary TopicSurgery/Transplantation
Model Rat
Tertiary TopicTransplantation/Graft Injury
Vehicle Solution (Electrolysis)
pH Neutral
Application Immersion