Hydrogen-Rich Saline Provides Protection Against Hyperoxic Lung Injury

Hengyi Tao, Jianmei Cai, Qiang Sun, Shulin Liu, Weigang Xu, Xue-Jun Sun, Yun Liu

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DOI: 10.1016/j.jss.2010.09.024 DOI is the universal ID for this study.

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Hydrogen has been proven to be a novel antioxidant through its selectively reducing of the hydroxyl radical. In this study, we investigated the effects of hydrogen-rich saline on the prevention of acute lung injury induced by hyperoxia (HALI) in rats. Physiologic saline, hydrogen-rich saline, or nitrogen-rich saline was administered through intraperitoneal (i.p.) injection during exposure to hyperoxia (10 mL/Kg), respectively. Severity of HALI was assessed by the volume of pleural effusion, wet-to-dry weight ratio (W/D), and histologic analysis. Apoptosis in lung cells was determined with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive staining. The content of pro-inflammatory cytokine interleukin IL-1b and TNF-a in the lung tissues were detected by enzyme-linked immunosorbent assay (ELISA). Hydrogen-rich saline treatment provides protection against HALI by inhibiting lipid, DNA oxidation, and tissue edema. Moreover, hydrogen-rich saline treatment could inhibit apoptosis and inflammation while no significant reduction was observed in nitrogen-rich saline treated animals. The results of this study demonstrate that hydrogen-rich saline ameliorated hyperoxia-induced acute lung injury by reducing oxidative stress and inflammatory cascades in lung tissue.

Publish Year 2011
Country China
Rank Positive
Journal Journal of Surgical Research
Primary Topic Lung
Secondary TopicSurgery/Transplantation
Model Rat
Tertiary TopicHyperoxia
Vehicle Saline (Dissolved)
pH Neutral
Application Injection