Hydrogen-rich saline attenuates skin ischemia/reperfusion induced apoptosis via regulating Bax/Bcl-2 ratio and ASK-1/JNK pathway

Ai-Xin Cheng, Fei Xie, Jia-La Li, Ming-Zhi Zhang, Peng-Xiang Zhao, Ting-Ting Wang, Xin Zhang, Xue-Mei Ma, Yi-Ding Xiao, Yi-Fang Liu, You-Bin Wang, Yun-Qi Liu

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DOI: 10.1016/j.bjps.2015.03.001 DOI is the universal ID for this study.

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Many pathways have been reported involving the effect of hydrogen-rich saline on protecting skin flap partial necrosis induced by the inflammation of ischemia/reperfusion injury. This study focused on the influence of hydrogen-rich saline treatment on apoptosis pathway of ASK-1/JNK and Bcl-2/Bax radio in I/R injury of skin flaps. Adult male Sprague-Dawley rats were divided into three groups. Group 1 was sham surgery group, Group 2 and 3 were ischemia/reperfusion surgery treated with physiological saline and hydrogen-rich saline respectively. Blood perfusion of flap was measured by Laser doppler flowmeters. Hematoxylin and eosin staining was used to observe morphological changes. Early apoptosis in skin flap was observed through TUNEL staining and presented as the percentage of TUNEL-positive cells of total cells. pASK-1, pJNK, Bcl-2 and Bax were examined by immunodetection. In addition Bcl-2, Bax and caspase-3 were detected by qPCR. Caspase-3 activity was also measured. Compared to the Group 2, tissues from the group 3 were observed with a high expression of Bcl-2 and a low expression of pASK-1, pJNK, and Bax, a larger survival area and a high level of blood perfusion. Hydrogen-rich saline ameliorated inflammatory infiltration and decreased cell apoptosis. The results indicate that hydrogen-rich saline could ameliorate ischemia/reperfusion injury and improve flap survival rate by inhibiting the apoptosis factor and, at the same time, promoting the expression of anti-apoptosis factor. Copyright © 2015. Published by Elsevier Ltd.

Publish Year 2015
Country China
Rank Positive
Journal Journal of Plastic, Reconstructive, and Aesthetic Surgery
Primary Topic Skin
Secondary TopicSurgery/Transplantation
Model Rat
Tertiary TopicPlastic Surgery
Vehicle Saline (Dissolved)
pH Neutral
Application Injection