Nano-Bubble Hydrogen Water Shows Superior Antioxidant Power vs Regular H2
- Authors
- Shinya Kato, Daigo Matsuoka, Nobuhiko Miwa
- Journal
- Materials Science and Engineering C
- Year
- 2015
- DOI
- 10.1016/j.msec.2015.03.064
- Study Type
- Molecular Assay
- Outcome
- Positive
- Peer Reviewed
- Yes
- Country
- Japan
- Health Condition
- Oxidative Stress
- Body System
- Whole Body
TL;DR
Nano-bubble hydrogen water is better at neutralizing harmful molecules than other types of hydrogen water, even when they have similar hydrogen levels.
Key Finding
Nano-bubble hydrogen water showed superior hydroxyl radical-scavenging activity compared to conventional hydrogen water and pure water in laboratory tests, with the nano-bubble structure appearing more important than dissolved hydrogen concentration alone.
Summary
Researchers created water containing tiny hydrogen bubbles (smaller than 717 nanometers) and tested whether it could neutralize harmful molecules called hydroxyl radicals. Using two different laboratory tests, they compared this nano-bubble hydrogen water to regular water, tap water, and other types of hydrogen water. The nano-bubble version showed the strongest ability to neutralize hydroxyl radicals and demonstrated antioxidant activity (the ability to prevent damage from oxidation), suggesting that the bubble structure itself may be more important than just the amount of dissolved hydrogen.
Practical Takeaway
This is a laboratory study only—it does not involve human testing or real-world conditions. While the results suggest nano-bubble hydrogen water may have stronger antioxidant properties in test tubes than other types of hydrogen water, this does not yet demonstrate health benefits in people. Much more research, including human trials, would be needed before any health claims could be made.
Abstract
We prepared nano-bubble hydrogen-dissolved water (nano-H water) which contained hydrogen nano-bubbles of <717-nm diameter for 54% of total bubbles. In the DMPO-spin trap electron spin resonance (ESR) method, the DMPO-OH:MnO ratio, being attributed to amounts of hydroxyl radicals (OH), was 2.78 for pure water (dissolved hydrogen [DH]≤0.01ppm, oxidation-reduction potential [ORP]=+324mV), 2.73 for tap water (0.01ppm, +286mV), 2.93 for commercially available hydrogen water (0.075ppm, +49mV), and 2.66 for manufactured hydrogen water (0.788ppm, -614mV), whereas the nano-H water (0.678ppm, -644mV) exhibited 2.05, showing the superiority of nano-H water to other types of hydrogen water in terms of OH-scavenging activity. Then, the reduction activity of nano-H water was assessed spectrophotometrically by the 2,2'-bipyridyl method. Differential absorbance at 530nm was in the order: 0.018 for pure water, 0.055 for tap water, 0.079 for nano-H water, 0.085 for commercially available hydrogen water, and 0.090 for manufactured hydrogen water, indicating a prominent reduction activity of hydrogen water and nano-H water against oxidation in ascorbate-coupled ferric ion-bipyridyl reaction. Thus, nano-H water has an improved antioxidant activity as compared to hydrogen water of similar DH-level, indicating the more marked importance of nano-bubbles rather than the concentration of hydrogen in terms of OH-scavenging. Copyright © 2015 Elsevier B.V. All rights reserved.