Hydrogen Water Prevents Cataracts in Animal Study by 50%
- Authors
- Yingxin Qu, Xiaoqi Li, Qinghua Yang, Runpu Li, Ye Tao, Yifei Huang, Liqiang Wang
- Journal
- International Ophthalmology
- Year
- 2026
- DOI
- 10.1007/s10792-025-03915-6
- Study Type
- Rat
- Outcome
- Positive
- Peer Reviewed
- Yes
- Country
- China
- Health Condition
- Cataracts
- Body System
- Ocular
TL;DR
Hydrogen-rich saline reduced cataract formation and protected lens clarity by lowering oxidative stress and supporting normal cellular metabolism.
Key Finding
Hydrogen-rich saline reduced cataract incidence to 50% in treated rats versus 100% in untreated controls, with proteomic analysis showing the treatment altered stress-response and metabolic proteins in the lens.
Summary
Researchers tested whether hydrogen-rich saline (salt water containing dissolved hydrogen) could prevent cataracts in rats exposed to a cataract-causing chemical. They found that hydrogen-rich saline reduced cataract development to 50% compared to 100% in untreated rats, and identified specific proteins in the lens that changed when hydrogen-rich saline was used, suggesting it works by reducing oxidative stress (cellular damage from unstable molecules).
Practical Takeaway
This rat study provides early evidence that hydrogen-rich saline may help prevent cataracts through antioxidant mechanisms, but results from animal studies do not automatically apply to humans. Further research in human subjects would be needed to determine if hydrogen water or hydrogen-rich saline could offer similar protective effects for cataract prevention in people.
Abstract
Purpose: Cataracts are associated with oxidative stress-induced damage to lens proteins. This study aims to identify differentially expressed proteins (DEPs) associated with the protective effects of hydrogen-rich saline (HRS) against N-methyl-N-nitrosourea (MNU)-induced cataracts, utilizing the antioxidant properties of hydrogen. Methods: Sprague-Dawley rats were assigned to control, MNU-only, MNU + normal saline (NS), MNU + pirenoxine(PRX), and MNU + HRS groups. Cataracts were induced with MNU (postnatal day 15), and treatments (postnatal days 8-21) included intraperitoneal injections and eye drops. Cataract severity was assessed using slit-lamp examinations, Pentacam analysis, and spectrophotometry. Proteomic analysis of lens tissues from the MNU + HRS and MNU + NS groups employed tandem mass tag (TMT) labeling and mass spectrometry. DEPs were identified, grouped based on fold changes, and analyzed for Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG),, and domain enrichment. Parallel reaction monitoring (PRM) validated selected DEPs. Results: HRS reduced MNU-induced cataract incidence to 50% versus 100% in MNU-only and NS groups and preserved lens clarity comparable to normal controls. Proteomic analysis identified 90 upregulated and 303 downregulated proteins in the HRS-treated group versus the NS-treated group. DEPs were enriched in GO terms related to ion transport, homeostasis, and ATP hydrolysis, as well as KEGG pathways like oxidative phosphorylation and arginine biosynthesis. Domain enrichment showed links to ATPase activity and energy metabolism. DEPs were grouped into Q1-Q4, with Q1 showing enrichment in oxidative phosphorylation and metabolic pathways. PRM confirmed the downregulation of 14 stress-response and metabolic proteins in the HRS-treated group. Conclusion: HRS mitigates MNU-induced cataracts possibly by reducing oxidative stress and downregulating stress-response and metabolic proteins.