Hydrogen Water Reduces Intestinal Damage and Blood Clotting Issues in Rats
- Authors
- Ling Yang, Yan Guo, Xin Fan, Ye Chen, Bo Yang, Ke-Xuan Liu, Jun Zhou
- Journal
- Mediators of Inflammation
- Year
- 2020
- DOI
- 10.1155/2020/4359305
- Study Type
- Rat
- Outcome
- Positive
- Peer Reviewed
- Yes
- Country
- China
- Health Condition
- Intestinal Ischemia-Reperfusion Injury
- Body System
- Digestive System
TL;DR
Hydrogen-rich solution (HRS) treatment improves survival and reduces blood clotting issues and inflammation in rats with intestinal damage due to restricted and then restored blood flow.
Key Finding
Hydrogen-rich solution improved survival rates and reduced intestinal damage, inflammation, and coagulation disorders in rats with intestinal ischemia/reperfusion injury.
Summary
This rat study tested whether hydrogen-rich solution could help reduce damage from intestinal ischemia/reperfusion injury—a condition where blood flow to the intestines is blocked and then restored, causing inflammation and blood clotting problems. Researchers gave rats either hydrogen-rich solution or regular saline before allowing blood flow to return to their intestines. Rats receiving hydrogen-rich solution had better survival rates, less intestinal damage, and reduced inflammation and blood clotting abnormalities compared to the control group.
Practical Takeaway
This early animal study suggests hydrogen water may help protect against intestinal damage from lack of blood flow, but these results are from rats only and have not been tested in humans. Much more research, including human trials, would be needed before any conclusions could be drawn about hydrogen water's effects in people with similar conditions.
Abstract
Intestinal ischemia/reperfusion (I/R) injury often causes inflammatory responses and coagulation disorders, which is further promoting the deterioration of the disease. Hydrogen has anti-inflammatory, antioxidative, and antiapoptotic properties against various diseases. However, the effect of hydrogen on coagulation dysfunction after intestinal I/R and the underlying mechanism remains unclear. The purpose of this study was to explore whether hydrogen-rich solution (HRS) could attenuate coagulation disorders and inflammation to improve intestinal injury and poor survival following intestinal I/R. The rat model of intestinal I/R injury was established by clamping the superior mesenteric artery for 90 min and reperfusion for 2 h. HRS (10 or 20 mL/kg) or 20 mL/kg 0.9% normal saline was intravenously injected at 10 min before reperfusion, respectively. The samples were harvested at 2 h after reperfusion for further analyses. Moreover, the survival rate was observed for 24 h. The results showed that HRS improved the survival rate and alleviated serum diamine oxidase activities, intestinal injury, edema, and apoptosis. Interestingly, HRS markedly improved intestinal I/R-mediated coagulation disorders as evidenced by abnormal conventional indicators of coagulation and thromboelastography. Additionally, HRS attenuated inflammatory responses and the elevated tissue factor (TF) and inhibited nuclear factor kappa beta (NF-κB) and nucleotide binding and oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome activation in peripheral blood mononuclear cells. Moreover, inflammatory factors and myeloperoxidase were closely associated with TF level. This study thus emphasized upon the amelioration of coagulation disorders and inflammation by HRS as a mechanism to improve intestinal I/R-induced intestinal injury and poor survival, which might be partially related to inhibition of NF-κB/NLRP3 pathway.