All Studies

Hydrogen-rich water prevents progression of nonalcoholic steatohepatitis and accompanying hepatocarcinogenesis in mice

Oxidative stress is a strong contributor to the progression from simple fatty liver to nonalcoholic steatohepatitis (NASH). Molecular hydrogen is an effective antioxidant that reduces cytotoxic reactive oxygen species. In this study, we investigated the effects of hydrogen-rich water and the drug…

Hydrogen: From a Biologically Inert Gas to a Unique Antioxidant

Protective effect of hydrogen-lactated Ringer's solution against extensive burn-induced intestine injury in rats after delayed fluid resuscitation

Objective To investigate the protective effect of hydrogen-rich lactated Ringer’s solution (HRS) against intestine injury induced by extensive burn in Sprague-Dewley (SD) rat model after delayed fluid resuscitation. Methods Thirty-six male SD rats were randomly divided into three groups (n=12)…

Hydrogen-rich saline alleviates early brain injury via reducing oxidative stress and brain edema following experimental subarachnoid hemorrhage in rabbits

Abstract Background Increasing experimental and clinical data indicate that early brain injury (EBI) after subarachnoid hemorrhage (SAH) largely contributes to unfavorable outcomes, and it has been proved that EBI following SAH is closely associated with oxidative stress and brain edema…

Hydrogen-rich saline reverses oxidative stress, cognitive impairment, and mortality in rats submitted to sepsis by cecal ligation and puncture

Background: Sepsis is associated with high morbidity and mortality, and survivors can present with cognitive dysfunction. The present study was performed to investigate the effects of hydrogen-rich saline (HRS) on oxidative stress in the brain, cognitive dysfunction, and mortality in a rat model…

Chronic hydrogen-rich saline treatment reduces oxidative stress and attenuates left ventricular hypertrophy in spontaneous hypertensive rats

In hypertensive animals and patients, oxidative stress represents the primary risk factor for progression of left ventricular hypertrophy. Recently, it has been demonstrated that hydrogen, as a novel antioxidant, can selectively reduce hydroxyl radicals and peroxynitrite anion to exert therapeutic…

Combination therapy with molecular hydrogen and hyperoxia in a murine model of polymicrobial sepsis

Sepsis is the most common cause of death in intensive care units. Some studies have found that hyperoxia may be beneficial to sepsis. However, the clinical use of hyperoxia is hindered by concerns that it could exacerbate organ injury by increasing free radical formation. Recently, it has been…

Profiling molecular changes induced by hydrogen treatment of lung allografts prior to procurement

We previously demonstrated that donor treatment with inhaled hydrogen protects lung grafts from cold ischemia/reperfusion (I/R) injury during lung transplantation. To elucidate the mechanisms underlying hydrogen's protective effects, we conducted a gene array analysis to identify changes in gene…

Hydrogen saline is protective for acute lung ischaemia/reperfusion injuries in rats

Protective effects of saturated hydrogen (H(2)) saline on cardiac ischaemia-reperfusion (I/R) injury have been demonstrated previously. This study was designed to show that hydrogen-rich saline is protective in preventing lung I/R injury in rats. Adult male Sprague-Dawley rats underwent 45 min…

Inhalation of hydrogen gas protects against myocardial stunning and infarction in swine

The present study was carried out to determine whether inhalation of hydrogen (H(2)) gas protects myocardium against ischemia-reperfusion (I/R) injury in swine. In anesthetized open-chest swine, myocardial stunning was produced by 12-minute occlusion of left anterior descending coronary artery…

Does alkaline‐reduced hexagonal water delay the aging process in<i>Drosophila</i>?

Hydrogen-rich saline reduces the oxidative stress and relieves the severity of trauma-induced acute pancreatitis in rats

Currently, little evidence exists to support whether the therapeutic approaches for treating ordinary acute pancreatitis (AP) are effective in trauma-induced pancreatitis. Hydrogen-rich (H2) saline is an antioxidant treatment capable of ameliorating the severity of L-arginine-induced AP. In this…

Electrolyzed-reduced water confers increased resistance to environmental stresses

Electrolysis of water produces reduced water at the cathode and oxidized water at the anode. Electrolyzed-reduced water (ERW) has an extremely negative oxidation-reduction potential. ERW scavenges cellular reactive oxygen species (ROS) and suppresses single-strand breaks of plasmid DNA in bacteria…

Hydrogen-supplemented drinking water protects cardiac allografts from inflammation-associated deterioration

Recent evidence suggests that molecular hydrogen has therapeutic value for disease states that involve inflammation. We hypothesized that drinking hydrogen-rich water (HW) daily would protect cardiac and aortic allograft recipients from inflammation-associated deterioration. Heterotopic heart…

Effect of Hydrogen Gas on the Survival Rate of Mice Following Global Cerebral Ischemia.

Global cerebral ischemia and reperfusion (I/R) often result in high mortality. Free radicals have been reported to play an important role in global cerebral I/R, and therefore, reduction of these might improve the outcome. Here, we investigated the effect of hydrogen gas (H2) (a strong free radical…

Hydrogen-Rich Saline Attenuates Lung Ischemia-Reperfusion Injury in Rabbits

Hydrogen gas, an antioxidant agent, was found to protect against cerebral and myocardial ischemia-reperfusion (I/R) injury. In the present study, we investigated the effect of hydrogen-rich saline (HRS) on the I/R-induced lung injury. Left lung of male New Zealand White rabbits rendered…

Inhaled hydrogen gas therapy for prevention of testicular ischemia/reperfusion injury in rats

This study evaluated whether 2% hydrogen (H(2)) gas therapy protects against testicular ischemia/reperfusion injury which results in increased formation of reactive oxygen species and/or reactive nitrogen species, leading to testicular apoptosis and impaired spermatogenesis. Pubertal six-week-old…

Effect of Reconstituted Apple Juice with Alkaline Electrolyzed Water on Cell Proliferation and Apoptosis of HT-29 Cells

Apple juice, known as a good antioxidant source, may partially protect the body from oxidative stress. In this report, the effect of apple juice reconstituted with alkaline electrolyzed water (EO-apple juice) on HT-29 cells was investigated. Specifically, the total antioxidant capacity (ORAC…

Hydrogen-rich electrolyzed warm water represses wrinkle formation against UVA ray together with type-I collagen production and oxidative-stress diminishment in fibroblasts and cell-injury prevention in keratinocytes

Hydrogen-rich electrolyzed warm water (HW) was prepared at 41°C and exhibited dissolved hydrogen (DH) of 1.13 ppm and an oxidation-reduction potential (ORP) of -741 mV in contrast to below 0.01 ppm and+184 mV for regular warm water (RW). Fibroblasts OUMS-36 and keratinocytes HaCaT were used…

Colloidal platinum in hydrogen-rich water exhibits radical-scavenging activity and improves blood fluidity

The 'colloidal platinum' stabilized with polyvinylpyrrolidone (Pt/PVP-colloid) was dispersed in hydrogen-rich water (HW; hydrogen concentration, 0.82 ppm; oxidation-reduction potential, -583 mV) or regular water (RW; <0.01 ppm, +218 mV). And we evaluated the antioxidant activity of Pt/PVP-colloid…