Alkaline Apple Juice and Cell Proliferation in Lab Cell Studies
Effect of Reconstituted Apple Juice with Alkaline Electrolyzed Water on Cell Proliferation and Apoptosis of HT-29 Cells
Research hubs: Digestive System · Immune System
This research was done on cells or tissue in a lab, not in people or animals.
- Evidence type
- Laboratory study (cells or tissue)
- Authors
- M. Kim, J. Fischer, Y.-C. Hung
- Journal
- Journal of Food and Drug Analysis
- Year
- 2012
- Study Type
- Cell Culture
- Delivery
- Water (Electrolysis)
- Outcome
- Positive
- Peer Reviewed
- Yes
- Cited by
- 1 (OpenAlex, as of November 2026)
- Country
- South Korea
TL;DR
Apple juice made with alkaline electrolyzed water has stronger antioxidant effects and is better at slowing down cancer cell growth than apple juice made with regular water.
Key Finding
Apple juice reconstituted with alkaline electrolyzed water showed higher antioxidant capacity and more effectively reduced colon cancer cell growth compared to apple juice made with regular purified water in laboratory tests.
Summary
Researchers tested whether apple juice mixed with alkaline electrolyzed water (water treated with electricity to increase alkalinity) had stronger antioxidant effects than apple juice mixed with regular purified water. Using laboratory-grown human colon cancer cells, they measured antioxidant strength and found that the electrolyzed water version had higher antioxidant capacity and was more effective at slowing cancer cell growth and triggering cell death.
Practical Takeaway
This is a laboratory cell study only—it does not involve humans or animals, so it cannot tell us whether these effects would occur in the body. While the results suggest alkaline electrolyzed water may enhance apple juice's antioxidant properties in test tubes, much more research would be needed before drawing any conclusions about health benefits for cancer prevention or treatment.
Abstract (excerpt)
Apple juice, known as a good antioxidant source, may partially protect the body from oxidative stress. In this report, the effect of apple juice reconstituted with alkaline electrolyzed water (EO-apple juice) on HT-29 cells was investigated. Specifically, the total antioxidant capacity (ORAC…