Hydrogen Gas Treatment Alters Molecular Changes in Rat Lung Allografts
Profiling molecular changes induced by hydrogen treatment of lung allografts prior to procurement
Research hubs: Respiratory System
This research was done in animals. Results in animals often don't carry over to people.
- Evidence type
- Animal study (rat)
- Authors
- Yugo Tanaka, Norihisa Shigemura, Tomohiro Kawamura, Kentaro Noda, Kumiko Isse, Donna Beer Stolz, Timothy R. Billiar, Yoshiya Toyoda, Christian A. Bermudez, James Lyons-Weiler, Atsunori Nakao
- Journal
- Biochemical and Biophysical Research Communications
- Year
- 2012
- PMID
- 22902635
- Study Type
- Rat
- Delivery
- Gas
- Dose
- 2% hydrogen inhalation for 3 hours before harvest
- H₂ concentration
- 2% H2 gas
- Outcome
- Positive
- Peer Reviewed
- Yes
- Cited by
- 25 (OpenAlex, as of November 2026)
- Country
- United States
TL;DR
Breathing in hydrogen gas helps protect donated lungs from damage during transplant by changing the activity of certain protective genes.
Key Finding
Hydrogen gas treatment of donor lungs before transplantation activated 229 genes, including those involved in lung surfactant production, cellular energy (ATP) generation, and stress-response proteins like HSP70, which may protect grafts from transplant-related injury.
Summary
Researchers exposed donor rat lungs to hydrogen gas before transplantation to understand how hydrogen protects organs from damage during the transplant process. They analyzed which genes were turned on or off in the hydrogen-treated lungs and found that hydrogen activated genes related to lung surfactant (a substance that helps lungs function), energy production, and stress protection. These molecular changes appeared to help the lungs survive the cold storage and transplant procedure better.
Practical Takeaway
This is a laboratory study in rats examining the molecular mechanisms of hydrogen's protective effects on transplanted lungs—not a study of hydrogen water for human health. While the findings suggest hydrogen may help preserve organ function during transplantation, this research does not provide evidence about hydrogen water's effects on healthy individuals or disease prevention.
Abstract (excerpt)
We previously demonstrated that donor treatment with inhaled hydrogen protects lung grafts from cold ischemia/reperfusion (I/R) injury during lung transplantation. To elucidate the mechanisms underlying hydrogen's protective effects, we conducted a gene array analysis to identify changes in gene…