Hydrogen-Rich Saline Provides Protection Against Hyperoxic Lung Injury
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DOI:
10.1016/j.jss.2010.09.024
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Abstract:
Hydrogen has been proven to be a novel antioxidant through its selectively reducing of the hydroxyl radical. In this study, we investigated the effects of hydrogen-rich saline on the prevention of acute lung injury induced by hyperoxia (HALI) in rats. Physiologic saline, hydrogen-rich saline, or nitrogen-rich saline was administered through intraperitoneal (i.p.) injection during exposure to hyperoxia (10 mL/Kg), respectively. Severity of HALI was assessed by the volume of pleural effusion, wet-to-dry weight ratio (W/D), and histologic analysis. Apoptosis in lung cells was determined with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive staining. The content of pro-inflammatory cytokine interleukin IL-1b and TNF-a in the lung tissues were detected by enzyme-linked immunosorbent assay (ELISA). Hydrogen-rich saline treatment provides protection against HALI by inhibiting lipid, DNA oxidation, and tissue edema. Moreover, hydrogen-rich saline treatment could inhibit apoptosis and inflammation while no significant reduction was observed in nitrogen-rich saline treated animals. The results of this study demonstrate that hydrogen-rich saline ameliorated hyperoxia-induced acute lung injury by reducing oxidative stress and inflammatory cascades in lung tissue.| Publish Year | 2011 |
|---|---|
| Country | China |
| Rank | Positive |
| Journal | Journal of Surgical Research |
| Primary Topic | Lung |
| Secondary Topic | Surgery/Transplantation |
| Model | Rat |
| Tertiary Topic | Hyperoxia |
| Vehicle | Saline (Dissolved) |
| pH | Neutral |
| Application | Injection |
| Comparison | |
| Complement |