Molecular hydrogen attenuates radiation-induced nucleobase damage to DNA in aerated aqueous solutions

Bernard Gardette, Bouchra Gharib, Christian Triantaphylides, Jean Cadet, Max De Reggi, Mhamad Abou-Hamdan, Thierry Douki

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DOI: 10.1080/09553002.2016.1206234 DOI is the universal ID for this study.

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Purpose: The main aim of the present study is to gain mechanistic insights into the modulating effect of molecular hydrogen on the γ-radiation-induced alteration pathways of DNA nucleobases.

Materials and methods: Aerated aqueous solutions of calf thymus DNA were exposed to a (60)Co source at doses ranging from 0 to 55 Gy under normoxic conditions, in the presence or not of 0.7 MPa hydrogen or helium. The measurement of several modified bases was performed using HPLC associated with electrospray ionization tandem pass spectrometry (HPLC-ESI-MS/MS). Bleaching of aqueous solutions of p-nitrosodimethylaniline (p-NDA) solutions was also used to allow the quantification of hydroxyl radical (•OH) formation.

Results: pNDA bleaching was significantly reduced in the presence of hyperbaric hydrogen. This is undoubtedly due to (•)OH scavenging by H2 since, under the same conditions, He had no effect. Similarly, base alterations were significantly reduced in the presence of hydrogen, as compared to controls under normal atmosphere or in the presence of helium. The relative proportions of modified nucleobases were not changed, showing that the only effect of H2 is to scavenge (•)OH without exhibiting reducing properties. Conclusions: Our findings demonstrate that H2 exerts a significant protection against radiation-induced DNA base damage in aqueous solutions, (•)OH scavenging being the only mechanism involved.

Publish Year 2016
Country France
Rank Positive
Journal International Journal of Radiation Biology
Primary Topic Whole Body
Secondary TopicRadioprotection
Model Cell Culture
Tertiary TopicGamma Radiation
Vehicle Water (Dissolved)
pH Neutral
Application Culture Media
Comparison Helium