Hydrogen Water Protects Heart from Damage During Heart Attacks in Rats
- Authors
- Liangtong Li, Tongtong Liu, Li Liu, Zhe Zhang, shaochun Li, Zhiling Zhang, Yujuan Zhou, Fulin Liu
- Journal
- Journal of Bioenergetics and Biomembranes
- Year
- 2020
- DOI
- 10.1007/s10863-020-09835-7
- Study Type
- Rat
- Outcome
- Positive
- Peer Reviewed
- Yes
- Country
- China
- Health Condition
- Myocardial Ischemia-Reperfusion Injury
- Body System
- Cardiovascular
TL;DR
Drinking hydrogen-rich water may help protect the heart from damage caused by temporary loss and restoration of blood flow.
Key Finding
Hydrogen-rich water altered metabolic pathways in rat heart tissue and changed the levels of key biomarkers involved in cell membrane function, suggesting a potential protective mechanism against ischemia-reperfusion injury.
Summary
Researchers studied whether hydrogen-rich water could protect rat hearts from damage caused by temporarily cutting off blood supply and then restoring it (a condition called ischemia-reperfusion injury). Using isolated rat hearts in a laboratory setup, they compared hearts treated with hydrogen-rich water to untreated controls and analyzed the chemical changes in heart tissue. The hydrogen-rich water appeared to alter several metabolic pathways (the chemical processes cells use for energy and survival) and changed the levels of certain fat-like molecules in the heart tissue, suggesting it may help reduce injury from blood flow interruption.
Practical Takeaway
This is an early-stage animal study using isolated rat hearts in a laboratory setting, not living organisms or humans. While the results suggest hydrogen-rich water may influence heart metabolism in ways that could be protective, much more research—including human studies—would be needed before drawing any conclusions about health benefits. The findings are too preliminary to guide consumer decisions.
Abstract
To investigate the effect of hydrogen-rich water on myocardial tissue metabolism in a myocardial ischemia-reperfusion injury (MIRI) rat model. Twelve rats were randomly divided into a hydrogen-rich water group and a control group of size 6 each. After the heart was removed, it was fixed in the Langendorff device, and the heart was perfused with 37 °C perfusion solution pre-balanced with oxygen. The control group was perfused with Kreb's-Ringers (K-R) solution, and the hydrogen-rich water group was perfused with K-R solution + hydrogen-rich water. Liquid Chromatograph Mass Spectrometer (LC-MS) analysis platform was used for metabolomics research. Principle component analysis (PCA), partial least squares discriminant analysis (PLS-DA), orthogonal partial least squares discriminant analysis (OPLS-DA), Variable importance in projection (VIP) value of OPLS-DA model (threshold value ≥1) were employed with independent sample T Test (p < 0.05) to find differentially expressed metabolites, and screen for differential metabolic pathways. VIP (OPLS-DA) analysis was performed with T test, and the metabolites of the control group and the hydrogen-rich water group were significantly different, and the glycerophospholipid metabolism was screened. Seven myocardial ischemia-reperfusion injury (MIRI)-related signaling pathways were identified, including glycerophospholipid metabolism, glycosylphosphatidylinositol (GPI) anchored biosynthesis, and purine metabolism, as well as 10 biomarkers such as phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine. Hydrogen-rich water regulates the metabolic imbalance that could change MIRI myocardial tissue metabolism, and alleviate ischemia-reperfusion injury in isolated hearts of rats through multiple signaling pathways.