Hydrogen Water Protects Against Heavy Metal Toxicity in Earthworms
- Authors
- Mine Köktürk, Serkan Yıldırım, Gizem Eser, Menekşe Bulut, Duried Alwazeer
- Journal
- Biological Trace Element Research
- Year
- 2021
- DOI
- 10.1007/s12011-021-02908-7
- Study Type
- Earthworm (Eudrilus eugeniae)
- Outcome
- Positive
- Peer Reviewed
- Yes
- Country
- Turkey
- Health Condition
- Heavy Metal Toxicity
- Body System
- Whole Body
TL;DR
Hydrogen-rich water helps earthworms better withstand the harmful effects of nickel pollution in soil, leading to less damage in their bodies and more cocoon production.
Key Finding
Hydrogen-rich water reduced nickel-induced oxidative stress, inflammation, and DNA damage in earthworms, while also improving cocoon production compared to nickel exposure alone.
Summary
Researchers exposed earthworms to nickel (a toxic heavy metal) in soil and tested whether hydrogen-rich water could protect them from damage. They found that earthworms given hydrogen-rich water showed less cellular damage, lower levels of harmful molecules called reactive oxygen species, reduced inflammation markers, and less DNA damage compared to earthworms exposed to nickel without hydrogen-rich water. The earthworms treated with hydrogen-rich water also produced more cocoons (their reproductive structures).
Practical Takeaway
This is an early-stage animal study in earthworms, not humans, so its relevance to human health is unknown. While the results suggest hydrogen-rich water may have protective properties against heavy metal toxicity in this model organism, much more research—including human studies—would be needed before drawing any conclusions about health benefits for people.
Abstract
In recent years, studies investigating the protective effect of hydrogen-rich water (HRW) against different diseases and the toxicity of some substances have attracted increasing attention. Here, we assessed the effects of hydrogen-rich water on different nickel-induced toxic responses (reactive oxygen species (ROS), tumor necrosis factor-alpha (TNF-α), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) of stress responses, histopathological changes) and cocoon production in earthworm model. Earthworms were randomly divided into two main groups: water (W) group including control (CW: ultrapure water), 10 (10W), 200 (200W), and 500 (500W), and hydrogen-rich ultrapure water (HRW) group including control (CHRW: hydrogen-rich ultrapure water), 10 (10HRW), 200 (200HRW), and 500 (500HRW) mg of nickel chloride kg-1 soil for 14 days. We found that cocoon production was less affected by the nickel exposure of earthworms in the 500HRW group compared to the 500W group. The ROS levels in 200HRW and 500HRW groups were less than that of 200W and 500W, respectively. The epithelial degeneration, epithelial necrosis, and necrosis in muscle fibers in tissues of earthworm were less damaged in 200HRW and 500HRW groups compared to 200W and 500W, respectively. HRW groups significantly reduced the expression of 8-OHdG induced by nickel exposure and inflammatory cytokine response including TNF-α. The study showed that hydrogen-rich water could alleviate the toxic effects of nickel-induced oxidative and inflammatory damages in earthworms. The HRW treatment known for its cheap and eco-friendly propertıes without any negative effects on the ecosystem can be used as a green method for alleviating the toxification effects of heavy metals in contaminated soil and increasing cocoon production of earthworms.