Hydrogen-rich saline ameliorates the retina against light-induced damage in rats

Feng Xia, Jing An, Lei Zhang, Liang Tian, Yashino Sugita, Zuo-Ming Zhang

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DOI: 10.1186/2045-9912-3-19 DOI is the universal ID for this study.

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Oxidative reactions are thought to be a major cause of light-induced retinal degeneration. This study was designed to investigate the effects of hydrogen-rich saline (HRS) on the prevention and treatment of light-induced retinal injury in rats. Male Sprague--Dawley rats were divided randomly into three groups: light damage, HRS prevention (5 ml/kg, 30 min before intensive light exposure), and HRS treatment (5 ml/kg per day for 5 days, after intensive light exposure), respectively. The right eye of each rat was exposed to 5000 lux constant white light-emitting diode (LED) light for 3 h, and the left eye was covered to serve as the blank control. Electroretinograms were recorded 5 days later, and the thickness of the outer nuclear layer (ONL) was measured after hematoxylin and eosin (H&E) staining. The results showed that the electroretinogram b-wave amplitudes and the mean ONL thicknesses of rats were significantly greater in the HRS prevention (P < 0.001) and treatment (P < 0.001) groups than in the light damage. These results indicated that peritoneal injection of HRS provides protection and treatment against light-induced retinal degeneration in rats.

Publish Year 2013
Country China
Rank Positive
Journal Medical Gas Research
Primary Topic Eye
Secondary TopicRadioprotection
Model Rat
Tertiary TopicLight Damage
Vehicle Saline (Dissolved)
pH Neutral
Application Injection