Hydrogen Gas Boosts Stem Cell Survival After Freezing
Hydrogen gas improves proliferation and mitochondrial activity of human adipose-derived stem cells after cryopreservation
Research hubs: Oxidative Stress
- Evidence type
- Laboratory study (cells or tissue)
- Authors
- Koji Kimura, Yasuhiko Tabata
- Journal
- Regenerative Therapy
- Year
- 2024
- DOI
- 10.1016/j.reth.2024.08.004
- Study Type
- Cell Culture
- Outcome
- Positive
- Peer Reviewed
- Yes
- Country
- Japan
- Health Condition
- Cellular Damage
- Body System
- Cellular
This research was done on cells or tissue in a lab, not in people or animals.
TL;DR
Adding hydrogen gas to the preservation solution improves the survival and growth of human stem cells after they are thawed from being frozen.
Key Finding
Hydrogen gas during cryopreservation significantly improved the survival, growth, and metabolic function of human adipose-derived stem cells after thawing, primarily by reducing oxidative stress.
Summary
This laboratory study tested whether hydrogen gas could help human fat-derived stem cells survive the freezing and thawing process better. Researchers froze cells with and without hydrogen gas present, then thawed them and measured how well they recovered. Cells frozen with hydrogen gas showed better survival, grew faster, and had less cellular damage from oxidative stress (a type of chemical damage) compared to cells frozen without it.
Practical Takeaway
While this is an early-stage laboratory study in cells (not humans), it suggests hydrogen gas may have potential applications in preserving stem cells for medical treatments. However, much more research is needed to determine if these benefits would apply to actual clinical use, and this study tells us nothing about hydrogen water consumption for general health.
Abstract (excerpt)
The objective of this study is to evaluate the effect of hydrogen gas on the biological functions of human adipose-derived stem cells (hADSC) in cryopreservation. hADSC were cryopreserved by a commercial cell preservation solution in the presence of hydrogen gas. After cryopreservation at -80 °C…