Hydrogen-enriched water restoration of impaired calcium propagation by arsenic in primary keratinocytes

Chih-Hung Lee, Hsin-Su Yu, Tohru Yoshioka, Wei-Tai Yu, Yu-Ching Chiu

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DOI: 10.1016/j.jseaes.2013.07.007 DOI is the universal ID for this study.

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Endemic contamination of artesian water for drinking by arsenic is known to cause several human cancers, including cancers of the skin, bladder, and lungs. In skin, multiple arsenic-induced Bowen's disease (As-BD) can develop into invasive cancers after decades of arsenic exposure. The characteristic histological features of As-BD include full-layer epidermal dysplasia, apoptosis, and abnormal proliferation. Calcium propagation is an essential cellular event contributing to keratinocyte differentiation, proliferation, and apoptosis, all of which occur in As-BD. This study investigated how arsenic interferes calcium propagation of skin keratinocytes through ROS production and whether hydrogen-enriched water would restore arsenic-impaired calcium propagation. Arsenic was found to induce oxidative stress and inhibit ATP- and thapsigaragin-induced calcium propagation. Pretreatment of arsenic-treated keratinocytes by hydrogen-enriched water or beta-mercaptoethanol with potent anti-oxidative effects partially restored the propagation of calcium by ATP and by thapsigaragin. It was concluded that arsenic may impair calcium propagation, likely through oxidative stress and interactions with thiol groups in membrane proteins.

Publish Year 2013
Country Taiwan
Rank Positive
Journal Journal of Asian Earth Sciences
Primary Topic Skin
Secondary TopicEnvironmental Pollution
Model Cell Culture
Tertiary TopicMetal Toxicity (Arsenic)
Vehicle Medium (Dissolved)
pH Neutral
Application Culture Media