Electrolyzed-reduced water protects against oxidative damage to DNA, RNA, and protein
Eun Ju Park, Kun-Kul Ryoo, Mi-Young Lee, Yoon Bae Lee, Yoon Kyoung Kim
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DOI:
10.1385/abab:135:2:133
DOI is the universal ID for this study.
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Abstract:
The generation of reactive oxygen species is thought to cause extensive oxidative damage to various biomolecules such as DNA, RNA, and protein. In this study, the preventive, suppressive, and protective effects of in vitro supplementation with electrolyzed-reduced water on H2O2-induced DNA damage in human lymphocytes were examined using a comet assay. Pretreatment, cotreatment, and posttreatment with electrolyzed-reduced water enhanced human lymphocyte resistance to the DNA strand breaks induced by H2O2 in vitro. Moreover, electrolyzed-reduced water was much more effective than diethylpyrocarbonate-treated water in preventing total RNA degradation at 4 and 25 degrees C. In addition, electrolyzed-reduced water completely prevented the oxidative cleavage of horseradish peroxidase, as determined using sodium dodecyl sulfate-polyacrylamide gels. Enhancement of the antioxidant activity of ascorbic acid dissolved in electrolyzed-reduced water was about threefold that of ascorbic acid dissolved in nonelectrolyzed deionized water, as measured by a xanthine-xanthine oxidase superoxide scavenging assay system, suggesting an inhibitory effect of electrolyzedreduced water on the oxidation of ascorbic acid.
| Publish Year |
2006 |
| Country |
South Korea |
| Rank |
Positive |
| Journal |
Applied Biochemistry and Biotechnology |
| Primary Topic |
Whole Body |
| Secondary Topic | ROS-Scavenging |
| Model |
Cell Culture |
| Tertiary Topic | Oxidative Stress |
| Vehicle |
Water (Electrolysis) |
| pH |
Alkaline |
| Application |
Culture Media |
| Comparison |
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| Complement |
Vitamin C |
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