All Studies

Protective effect of hydrogen-rich water against gentamicin-induced nephrotoxicity in rats using blood oxygenation level-dependent MR imaging

Purpose: We assessed intrarenal oxygenation in gentamicin-induced nephrotoxicity (GIN) and the protective effect of hydrogen-rich water (HW) against GIN using blood oxygenation level-dependent magnetic resonance (MR) imaging. Materials and methods: We acquired T(2)*-weighted images (T(2)*WI) of 21…

Biological toxicity of acid electrolyzed functional water: Effect of oral administration on mouse digestive tract and changes in body weight

Objective: Acid electrolyzed functional water has been used in a variety of ways because of its antiseptic action. In the present study, we investigated both the systemic and gastrointestinal effects of ingesting acid electrolyzed functional water, from the perspective of its use in mouthwash…

Protective effect of hydrogen-rich saline on decompression sickness in rats

Introduction: Hydrogen (H2) has been reported to be effective in the treatment of oxidative injury, which plays an important role in the process of decompression sickness (DCS). This study was designed to test whether H2-rich saline (saline saturated with molecular hydrogen) protected rats against…

Hydrogen inhalation ameliorates lipopolysaccharide-induced acute lung injury in mice

Acute lung injury (ALI) is a serious illness, the incidence and mortality of which are very high. Free radicals, such as hydroxyl radicals (OH) and peroxynitrite (ONOO(-)), are considered to be the final causative molecules in the pathogenesis of ALI. Hydrogen, a new antioxidant, can selectively…

Inhalation of hydrogen gas attenuates cisplatin-induced ototoxicity via reducing oxidative stress

Cisplatin, an anticancer drug used extensively to treat a broad range of tumors, has strong ototoxic side effects induced by reactive oxygen species (ROS). Recently, it has been reported that hydrogen gas (H(2)) is a new antioxidant by selectively reducing hydroxyl radical, the most cytotoxic ROS…

H2 inhibits TNF-α-induced lectin-like oxidized LDL receptor-1 expression by inhibiting nuclear factor κB activation in endothelial cells

H(2) is a therapeutic antioxidant that can reduce oxidative stress. Oxidized low-density lipoprotein, which plays roles in atherosclerosis, may promote endothelial dysfunction by binding the cell-surface receptor LOX-1. LOX-1 expression can be upregulated by various stimuli, including TNF-α. Thus…

Hydrogen-Rich Saline Provides Protection Against Hyperoxic Lung Injury

Hydrogen has been proven to be a novel antioxidant through its selectively reducing of the hydroxyl radical. In this study, we investigated the effects of hydrogen-rich saline on the prevention of acute lung injury induced by hyperoxia (HALI) in rats. Physiologic saline, hydrogen-rich saline…

Hydrogen therapy attenuates irradiation-induced lung damage by reducing oxidative stress

Molecular hydrogen (H(2)) is an efficient antioxidant that diffuses rapidly across cell membranes, reduces reactive oxygen species (ROS), such as hydroxyl radicals and peroxynitrite, and suppresses oxidative stress-induced injury in several organs. ROS have been implicated in radiation-induced…

Protective effects of hydrogen-rich saline on monocrotaline-induced pulmonary hypertension in a rat model

Abstract Background Hydrogen-rich saline has been reported to have antioxidant and anti-inflammatory effects and effectively protect against organ damage. Oxidative stress and inflammation contribute to the pathogenesis and/or development of pulmonary hypertension. In this study, we investigated…

Protective effects of hydrogen-rich saline in preeclampsia rat model

Hydrogen has been reported as a novel antioxidant to selectively reduce levels of toxic reactive-oxygen species (ROS). We investigated the effects of hydrogen-rich saline on the prevention of oxidative injuries in N(omega)-nitro-L-arginine methyl ester (L-NAME) induced rat model of preeclampsia…

The melamine excretion effect of the electrolyzed reduced water in melamine-fed mice

Our hypothesis is that the intake of functional water, electrolyzed reduced water (ERW) can excrete melamine in body was evoked by melamine-tainted feed (MTF). To address this issue, we investigated the effect of ERW in MTF-mice model by way of body weight gain, incidence of urinary crystals…

Anti-inflammatory effect of hydrogen-rich saline in a rat model of regional myocardial ischemia and reperfusion

To study the possible anti-inflammatory effect of hydrogen-rich saline (H(2) saline) on rat hearts with regional myocardial ischemia and reperfusion (I/R). Sixty-six rats were equally randomized to three groups: sham-operated group, I/R group (control group) and I/R plus H(2) saline treatment…

Hydrogen saline offers neuroprotection by reducing oxidative stress in a focal cerebral ischemia-reperfusion rat model

Hydrogen gas is neuroprotective in cerebral ischemia animal models. In this study, we tested the neuroprotective effects of hydrogen saline, which is safe and easy to use clinically, in a rat model of middle cerebral artery occlusion (MCAO). Sprague-Dawley male rats weighting 250-280 g were divided…

Studies and observations on the health effects of drinking electrolyzed-reduced alkaline water

Municipal drinking water, prefiltered and treated by partial electrolysis, followed by collecting the cathodic water that is alkaline (pH 8.5 to 9.5), shows a high negative oxidative reductive potential (ORP) (-150 to -250 mV) compared to untreated tap water (+150 mV) as well as smaller molecular…

The neuroprotective effects of electrolyzed reduced water and its model water containing molecular hydrogen and Pt nanoparticles

Therapeutic effects of hydrogen in animal models of Parkinson's disease

Since the first description of Parkinson's disease (PD) nearly two centuries ago, a number of studies have revealed the clinical symptoms, pathology, and therapeutic approaches to overcome this intractable neurodegenerative disease. 1-methy-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)…

Hydrogen-Enriched Preservation Protects the Isogeneic Intestinal Graft and Amends Recipient Gastric Function During Transplantation

Inhaled hydrogen gas exerts antioxidant and anti-inflammatory effects in rat intestinal transplantation. Here, we investigated whether ex vivo donor organ treatment with dissolved hydrogen would prevent intestinal graft injury. Isogeneic intestinal transplantation was performed in Lewis rats…

Effects of hydrogen-rich water on abnormalities in a SHR.Cg-Leprcp/NDmcr rat - a metabolic syndrome rat model

Hydrogen (H2), a potent free radical scavenger, selectively reduces the hydroxyl radical, which is the most cytotoxic of the reactive oxygen species (ROS). An increase in oxygen free radicals induces oxidative stress, which is known to be involved in the development of metabolic syndrome…

Hydrogen Saline Treatment Attenuates Hyperoxia-Induced Retinopathy by Inhibition of Oxidative Stress and Reduction of VEGF Expression

Objective: Retinal neovascularization or retinopathy is a proliferative disorder of the retinal capillaries and is the primary cause of blindness. Some studies have shown that oxidative stress plays an important role in hyperoxia-induced retinal neovascularization. Previous reports have indicated…

Hydrogen supplemented air inhalation reduces changes of prooxidant enzyme and gap junction protein levels after transient global cerebral ischemia in the rat hippocampus

Transient global cerebral ischemia (TGCI) occurs during acute severe hypotension depriving the brain of oxygen and glucose for a short period of time. During reperfusion, several mechanisms can induce secondary neuronal damage, including the increased production of reactive oxygen species (ROS)…