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Acid Water Mouthwash Found Safe in Mouse Study

Biological toxicity of acid electrolyzed functional water: Effect of oral administration on mouse digestive tract and changes in body weight

Research hubs: Digestive System

This research was done in animals. Results in animals often don't carry over to people.

Scientific visualization of hydrogen therapy research for Biological toxicity of acid electrolyzed functional water: Effect of oral administration on mouse digestive tract and changes in body weight
Evidence type
Animal study (mouse)
Authors
Chiharu Morita, Tetsuya Nishida, Koichi Ito
Journal
Archives of Oral Biology
Year
2011
Study Type
Mouse
Participants
17
Delivery
Water (Electrolysis)
Dose
Free access to acid electrolyzed functional water as drinking water for 8 weeks
Duration
8 weeks
Outcome
Positive
Peer Reviewed
Yes
Cited by
44 (OpenAlex, as of November 2026)
Country
Japan

TL;DR

Drinking acid electrolyzed water, which has antiseptic properties, appears safe for mice and does not cause any noticeable health issues.

Key Finding

Acid electrolyzed functional water showed no systemic toxicity or significant effects on the digestive tract in mice over eight weeks, with no adverse changes in body weight or oral tissues.

Summary

Researchers gave mice acid electrolyzed functional water (a type of water created through an electrical process) to drink for eight weeks to test whether it was safe. They compared mice drinking this water to mice drinking regular tap water, measuring body weight, checking their mouths, and examining their teeth under a microscope. The mice drinking the special water showed no differences in weight gain, no damage to their mouth tissues, and no tooth surface roughness compared to the control group, though some minor tooth wear was observed.

Practical Takeaway

This mouse study suggests that acid electrolyzed functional water may be safe for use as a mouthwash, though the observation of minor tooth wear warrants attention. Because this research was conducted only in mice, human studies would be needed to confirm whether these safety findings apply to people. The study does not address whether hydrogen water specifically has any health benefits.

Abstract (excerpt)

Objective: Acid electrolyzed functional water has been used in a variety of ways because of its antiseptic action. In the present study, we investigated both the systemic and gastrointestinal effects of ingesting acid electrolyzed functional water, from the perspective of its use in mouthwash…

Read the full abstract on PubMed