Hypoxia-ischemia (HI) brain injury is a major cause of neuronal cell death especially apoptosis in the perinatal period. This study was designated to examine the effect of hydrogen therapy on apoptosis in an established neonatal HI rat pup model. Seven-day-old rat pups were subjected to left common carotid artery ligation and then 90 min hypoxia (8% oxygen at 37 C). Immediately after HI insult, ...

Cerebral hypoxia-ischemia (HI) represents a major cause of brain damage in the term newborn. This study aimed to examine the short and long-term neuroprotective effect of hydrogen saline (H(2) saline) using an established neonatal HI rat pup model. Seven-day-old rat pups were subjected to left common carotid artery ligation and then 90 min hypoxia (8% oxygen at 37 degrees C). H(2) saturated saline ...

We have proposed an active hydrogen reduced water theory that active hydrogen produced by electrolysis of water is stabilized in the form of hydrogenated metal nanocolloids in electrolyzed reduced water (ERW) and scavenges intracellular reactive oxygen species (ROS). Because various brain diseases are caused by oxygen stress, we examined the effect of ERW on oxidative stress-induced apoptois of ...

Electrolyzed-reduced water (ERW) produced by electrolyzing water in cathode side has the ability to scavenge reactive oxygen species (ROS). Here, we investigated the effect of ERW on oxidative stress-induced neural cell death by glutamate. When cell viability assay was performed using primary rat cerebral cortical culture as neural model, ERW suppressed neural cell death by glutamate. Furthermore, ...

During the 7.1-MPa hydrogen-helium-oxygen record human dive, we tested the hypothesis that the increased ambient pressure would alter the maximal muscle performance, specifically that breathing dense gas would lead to fatigue of the respiratory muscle. A group of hand muscles (adductor pollicis, AP) and the inspiratory muscles (IM) were studied in three professional divers. Maximal voluntary ...

Six commercial divers were investigated for neurological and psychosensorimotor responses during an open sea dive to 500 m with a hydrogen-helium-oxygen mixture containing 49% hydrogen. Results showed only moderate neurological symptoms of high-pressure nervous syndrome, whereas the narcotic effect of hydrogen was detectable, as investigated by psychosensorimotor tests. Nevertheless, the divers ...