Hydrogen therapy reduces apoptosis in neonatal hypoxia-ischemia rat model

Hengyi Tao, Jianmei Cai, John H. Zhang, Runping Li, Shigeo Ohta, Sun Qiang, Weigang Xu, Wen-Wu Liu, Xu Luo, Xuejun Sun, Zhimin Kang

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DOI: 10.1016/j.neulet.2008.05.077 DOI is the universal ID for this study.

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Hypoxia-ischemia (HI) brain injury is a major cause of neuronal cell death especially apoptosis in the perinatal period. This study was designated to examine the effect of hydrogen therapy on apoptosis in an established neonatal HI rat pup model. Seven-day-old rat pups were subjected to left common carotid artery ligation and then 90 min hypoxia (8% oxygen at 37 C). Immediately after HI insult, pups were placed into a chamber filled with 2% H(2) for 30 min, 60 min, or 120 min, respectively. 24 h after 2% H2 therapy, the pups were decapitated and brain injury was assessed by 2,3,5-triphenyltetrazoliumchloride (TTC), Nissl, and TUNEL staining, as well as caspase-3, caspase-12 activities in the cortex and hippocampus. H(2) treatment in a duration-dependent manner significantly reduced the number of positive TUNEL cells and suppressed caspase-3 and -12 activities. These results indicated H(2) administration after HI appeared to provide brain protection via inhibition of neuronal apoptosis. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

Publish Year 2008
Country China
Rank Positive
Journal Neuroscience Letters
Primary Topic Brain
Secondary TopicBrain Injury
Model Rat
Tertiary TopicHypoxia-Ischemia
Vehicle Gas
pH N/A
Application Inhalation