The protective effects of hydrogen on HO-1 expression in the brainafter focal cerebral ischemia reperfusion in rats

Lianshuang Zhang, Tongshen Liu, Wei Zhao, Xifeng Wang

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DOI: 10.3906/sag-1502-3 DOI is the universal ID for this study.

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Background/aim: The aim of this study was to investigate whether a hydrogen administration can produce neuroprotective effects after brain ischemia reperfusion in rats.

Materials and methods: A brain ischemia reperfusion injury was induced by a 2-h left middle cerebral artery occlusion (MCAO) using an intraluminal filament, followed by 46 h of reperfusion. A hydrogen-rich saline (1 mL/kg body weight i.p.) was administered at the beginning of reperfusion. Saline (1 mL/kg)-treated animals were used as the control. Sham-operated animals were also used. Subsequently, 48 h after the MCAO, histological alternations, heme oxygenase-1 (HO-1) expression, and levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the cerebral cortex and the hippocampus were examined.

Results: Hydrogen significantly alleviated brain tissue histological damage, promoted HO-1 expression, upregulated levels of SOD, and decreased the levels of MDA in brain tissue after the ischemia reperfusion injury.

Conclusion: The results suggest that the neuroprotective effects of hydrogen may be mediated by promoting HO-1 expression and attenuated the oxidative injury.

Publish Year 2016
Country China
Rank Positive
Journal Turkish Journal of Medical Sciences
Primary Topic Brain
Secondary TopicBrain Injury
Model Rat
Tertiary TopicIschemia-Reperfusion Injury
Vehicle Saline (Dissolved)
pH Neutral
Application Injection