The improvement of insulin level after hydrogen-rich water therapy in streptozotocin-induced diabetic rats

Budi Susatia, Ekowati Retnaningtyas, Siti Nur Arifah, Sri Rahayu Lestari

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DOI: 10.14202/vetworld.2022.182-187 DOI is the universal ID for this study.

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Background and aim: Water plays a pivotal role in the body. Alteration of the fluid balance promotes metabolic disorder, thus leading to the development of various diseases, such as diabetes mellitus (DM). Hydrogen-rich water (HW) is recognized as a novel antioxidant. This study aimed to investigate the role of HW on insulin, insulin receptor (IRs), and superoxide dismutase (SOD) levels in streptozotocin (STZ)-induced diabetic rats.

Materials and methods: A total of 30 male Wistar rats were randomly divided into five groups: Normal (N), DM rats, DM+metformin (DM+Met, 45 mg/kg body weight [BW]), DM+Met+HW, and DM+HW. DM rats were induced by feeding them a high-fat diet for 30 days and then injecting with repeated low doses of STZ (35 mg/kg BW) intraperitoneally. Fresh HW was administered orally and ad libitum for 14 days. Insulin, IRs, and SOD were observed in each group.

Results: HW therapy increased the level and expression of insulin and IRs. In addition, treatment with HW also elevated the SOD levels in the serum and liver. The study results indicated no significant differences between the administration of HW and metformin.

Conclusion: HW has antioxidant activity in STZ-induced DM rats, increasing insulin, IRs, and SOD.

Publish Year 2022
Country Indonesia
Rank Positive
Journal Veterinary World
Primary Topic Whole Body
Secondary TopicDiabetes
Model Rat
Tertiary TopicGlucose Metabolism
Vehicle Water (Electrolysis)
pH Neutral
Application Ingestion
Complement Metformin