Suppression of two-stage cell transformation by electrolyzed reduced water containing platinum nanoparticles
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DOI:
10.1007/1-4020-4457-7_16
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Abstract:
According to the two-stage cell transformation theory, cancer cells first receive initiation, which is mainly caused by DNA damage and then promotion, which enhance transformation. Since murine Balb/c 3T3 cells lose contact inhibition by cell transformation, the cells have been widely used for transformation experiments. Electrolyzed reduced water (ERW) is a health beneficial alkaline drinking water containing high concentration of dissolved hydrogen, and can scavenge intracellular reactive oxygen species (ROS). ERW contains a small amount of platinum (Pt) nano- particles as atomic hydrogen donor and ROS-scavenger. Therefore, ERW supplemented with Pt nanoparticles (ERW/Pt) can be considered as a model of strong ERW. Here, we report that ERW/Pt can prevent transformation of Balb/c 3T3 cells. ERW was prepared by electrolysis of 0.002 M NaOH. Balb/c 3T3 cells were treated with 3-methyl cholantrene (MCA) as an initiation compound, followed by the treatment with phorbol-12-myristate-13-acetate (PMA) as a promotion compound. The cell transformation induced by MCA/PMA was strongly suppressed by ERW/Pt treatment, especially at the stage of promotion. Analysis of intracellular ROS level showed that ERW/Pt could decrease excess intracellular ROS induced by PMA. These results suggested that ERW/Pt suppressed cell transformation at promotion stage by its ROS scavenging effect.| Publish Year | 2006 |
|---|---|
| Country | Japan |
| Rank | Positive |
| Journal | Animal Cell Technology: Basic and Applied Aspects |
| Primary Topic | Whole Body |
| Secondary Topic | Cancer |
| Model | Cell Culture |
| Tertiary Topic | Oncogenesis |
| Vehicle | Water (Electrolysis) |
| pH | Alkaline |
| Application | Culture Media |
| Comparison | |
| Complement | Platinum |