Safety of inhaled hydrogen gas in healthy mice

Alexis R. Cole, Ali Raza, Brian D. Polizzotti, Humera Ahmed, John N. Kheir, Nick Andrews, Robert F. Padera

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DOI: 10.4103/2045-9912.266988 DOI is the universal ID for this study.

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The purpose of this work was to determine the safety of inhaled hydrogen gas in healthy animals. Female mice were exposed to medical air with or without hydrogen gas (concentration 2.4%) for 72 hours (n = 25 mice/group). Mice underwent a standardized and validated neurobehavioral examination, SmithKline Beecham, Harwell, Imperial College, Royal London Hospital, Phenotype Assessment (SHIRPA) protocol, prior to and following the exposure period. Blood was withdrawn for serologic evaluation and all major organ tissues were evaluated histologically. The average hydrogen concentration within the chamber was 2.27%. Following exposure, there was no significant change in body weight in either group. Similarly, there was no significant change in the total SHIRPA score, although hydrogen-treated mice exhibited significantly lower spontaneous locomotor activity (P 0.05 for all). Hydrogen gas did not appear to cause significant adverse effects when administered to healthy mice for 72 hours, with the possible exception of decreased spontaneous locomotor activity. The study was approved by the Institutional Animal Care and Use Committee at Boston Children's Hospital, USA (approved number 18-01-3536) on January 25, 2018.

Publish Year 2019
Country United States
Rank Positive
Journal Medical Gas Research
Primary Topic Whole Body
Secondary TopicHydrogen Biology
Model Mouse
Tertiary TopicHydrogen Safety
Vehicle Gas
pH N/A
Application Inhalation