New Approach in Translational Medicine: Effects of Electrolyzed Reduced Water (ERW) on NF-κB/iNOS Pathway in U937 Cell Line under Altered Redox State

Alessio Ferrone, Alfredo Grilli, Antonia Patruno, Daniela Maria Pia Gatta, Fausto Croce, Lorenza Speranza, Maria Anna De Lutiis, Mario Felaco, Mirko Pesce, Sara Franceschelli

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DOI: 10.3390/ijms17091461 DOI is the universal ID for this study.

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It is known that increased levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) can exert harmful effects, altering the cellular redox state. Electrolyzed Reduced Water (ERW) produced near the cathode during water electrolysis exhibits high pH, high concentration of dissolved hydrogen and an extremely negative redox potential. Several findings indicate that ERW had the ability of a scavenger free radical, which results from hydrogen molecules with a high reducing ability and may participate in the redox regulation of cellular function. We investigated the effect of ERW on H₂O₂-induced U937 damage by evaluating the modulation of redox cellular state. Western blotting and spectrophotometrical analysis showed that ERW inhibited oxidative stress by restoring the antioxidant capacity of superoxide dismutase, catalase and glutathione peroxidase. Consequently, ERW restores the ability of the glutathione reductase to supply the cell of an important endogenous antioxidant, such as GSH, reversing the inhibitory effect of H₂O₂ on redox balance of U937 cells. Therefore, this means a reduction of cytotoxicity induced by peroxynitrite via a downregulation of the NF-κB/iNOS pathway and could be used as an antioxidant for preventive and therapeutic application. In conclusion, ERW can protect the cellular redox balance, reducing the risk of several diseases with altered cellular homeostasis such as inflammation.

Publish Year 2016
Country Italy
Rank Positive
Journal International Journal of Molecular Sciences
Primary Topic Whole Body
Secondary TopicCellular Redox State
Model Cell Culture
Tertiary TopicOxidative Stress
Vehicle Water (Electrolysis)
pH Alkaline
Application Culture Media