Molecular Hydrogen Promotes Adipose-derived Stem Cell Myogenic Differentiation via Regulation of Mitochondria

Er-Kai Pang, Lei Hou, Ming-Sheng Liu, Rang-Shan Gao, Wen-Yong Fei, Yang-Yang Hu, Yu-Cheng Zhang, Yu-Xia Yang

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DOI: 10.2174/1574888X17666220926115240 DOI is the universal ID for this study.

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Background: Acute skeletal muscle injuries are common physical or sports traumas. Cellular therapy has excellent potential for regeneration after skeletal muscle injury. Adipose-derived stem cells (ADSCs) are a more accessible type of stem cell. However, it has a low survival rate and differentiation efficiency in the oxidative stress-rich microenvironment after transplantation. Although molecular hydrogen (H2) possesses anti-inflammatory and antioxidant biological properties, its utility in mitochondrial and stem cell research has not been adequately explored. Objective: Revealing the role of H2 on Adipose-derived stem cells myogenic differentiation.

Methods: The protective effects of H2 in ADSCs were evaluated by MTT assay, live-dead cell staining, western blot analysis, immunofluorescence staining, confocal imaging, and transmission electron microscopy.

Results: An appropriate volume fraction of H2 significantly decreased mitochondrial reactive oxygen species (ROS) levels, increased the number of mitochondria, and promoted mitophagy, thus enhancing the survival and myogenic differentiation of ADSCs.

Conclusion: This study reveals the application potential of H2 in skeletal muscle diseases or other pathologies related to mitochondrial dysfunction.

Publish Year 2022
Country China
Rank Positive
Journal Current Stem Cell Research & Therapy
Primary Topic Whole Body
Secondary TopicHydrogen Biology
Model Cell Culture
Tertiary TopicStem Cell Differentiation
Vehicle Gas
pH N/A
Application Ventilation