Long-term and daily use of molecular hydrogen induces reprogramming of liver metabolism in rats by modulating NADP/NADPH redox pathways

Fei Xie, Jin He, Mengyu Liu, Pengxiang Zhao, Shiwen Ma, Xiaokang Zhang, Xue Jiang, Xuemei Ma, Yang Yi, Yao Mawulikplimi Adzavon

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DOI: 10.1038/s41598-022-07710-6 DOI is the universal ID for this study.

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Molecular hydrogen (H2) has emerged as a new therapeutic option in several diseases and is widely adopted by healthy people. However, molecular data to support therapeutic functions attributed to the biological activities of H2 remain elusive. Here, using transcriptomic and metabolomic approaches coupled with biochemistry and micro-CT technics, we evaluated the effect of long-term (6 months) and daily use of H2 on liver function. Rats exposed 2 h daily to H2 either by drinking HRW (H2 dissolved in H2O) or by breathing 4% H2 gas showed reduced lipogenesis and enhanced lipolysis in the liver, which was associated with apparent loss of visceral fat and brown adipose tissue together with a reduced level of serum lipids. Both transcripts and metabolites enriched in H2-treated rats revealed alteration of amino acid metabolism pathways and activation of purine nucleotides and carbohydrate biosynthesis pathways. Analysis of the interaction network of genes and metabolites and correlation tests revealed that NADP is the central regulator of H2 induced metabolic alterations in the liver, which was further confirmed by an increase in the level of components of metabolic pathways that require NADP as substrate. Evidence of immune response regulation activity was also observed in response to exposure to H2. This work is the first to provide metabolomic and transcriptomic data to uncover molecular targets for the effect of prolonged molecular hydrogen treatment on liver metabolism.

Publish Year 2022
Country China
Rank Positive
Journal Nature Scientific Reports
Primary Topic Liver
Secondary TopicImmune Regulation
Model Rat
Tertiary TopicMetabolic Regulation
Vehicle Water (Dissolved)
pH Neutral
Application Ingestion