Hydrogen Saline Treatment Attenuates Hyperoxia-Induced Retinopathy by Inhibition of Oxidative Stress and Reduction of VEGF Expression

John Zhang, Lina Huang, Shihong Zhao, Xue-Jun Sun

Read more:

DOI: 10.1159/000329600 DOI is the universal ID for this study.

This link will take you to the full study.


Retinal neovascularization or retinopathy is a proliferative disorder of the retinal capillaries and is the primary cause of blindness. Some studies have shown that oxidative stress plays an important role in hyperoxia-induced retinal neovascularization. Previous reports have indicated that hydrogen has a therapeutic, antioxidant activity by selectively reducing hydroxyl radicals. This study examined the therapeutic effect of hydrogen saline on retinopathy in an established mouse model of hyperoxia-induced retinopathy. Mouse pups were exposed to 75% O(2) from postnatal day 7 (P7) to P12. Hydrogen saline was administered by intraperitoneal injection (5 ml/kg) daily for 5 days. On P17, the pups were decapitated, and retinal neovascularization was assessed using fluorescence imaging and histopathological examination. Vascular endothelial growth factor (VEGF) expression was evaluated using real-time polymerase chain reaction and fluorescence immunohistochemistry. Oxidative stress was quantified based on the malondialdehyde (MDA) level. Hydrogen saline decreased retinal neovascularization, reduced the mRNA and protein expression of VEGF, and suppressed the MDA levels. Hydrogen saline may be a potential treatment for hyperoxia-induced retinopathy that acts via the inhibition of oxidative stress and the reduction of VEGF expression.

Publish Year 2011
Country China
Rank Positive
Journal Ophthalmic Research
Primary Topic Eye
Secondary TopicRetinal Injury
Model Mouse
Tertiary TopicHyperoxia
Vehicle Saline (Dissolved)
pH Neutral
Application Injection