Hydrogen Water Protects Vision in Eye Injury Study
- Authors
- Jing-Chuan Sun, Tao Xu, Qiao Zuo, Ruo-Bing Wang, Ai-Qing Qi, Wen-Luo Cao, Ai-Jun Sun, Xue-Jun Sun, Jiajun Xu
- Journal
- PLoS One
- Year
- 2014
- DOI
- 10.1371/journal.pone.0099299
- Study Type
- Rat
- Outcome
- Positive
- Peer Reviewed
- Yes
- Country
- China
- Health Condition
- Optic Nerve Injury
- Body System
- Visual System
TL;DR
Breathing in hydrogen gas helps protect eye nerve cells and maintain vision in rats after an eye injury.
Key Finding
Hydrogen-rich saline significantly increased survival of retinal ganglion cells and preserved visual function in rats with optic nerve crush injury, while reducing markers of oxidative stress.
Summary
Researchers tested whether hydrogen-rich saline could protect nerve cells in the eye after an optic nerve injury in rats. Rats that received hydrogen-rich saline daily for two weeks after the injury had more surviving retinal ganglion cells (the nerve cells that transmit visual information), less cell death, lower levels of oxidative stress (cellular damage from unstable molecules), and better visual function compared to rats that received regular saline.
Practical Takeaway
This rat study suggests hydrogen may have neuroprotective properties for optic nerve injuries, but these results cannot yet be applied to humans. Much more research, including human clinical trials, would be needed to determine if hydrogen-rich saline could help prevent vision loss in people with optic nerve damage.
Abstract
Objective: To investigate the effect of molecular hydrogen (H2) in a rat model subjected to optic nerve crush (ONC). Methods: We tested the hypothesis that after optic nerve crush (ONC), retinal ganglion cell (RGC) could be protected by Hâ‚‚. Rats in different groups received saline or hydrogen-rich saline every day for 14 days after ONC. Retinas from animals in each group underwent measurements of hematoxylin and eosin (H&E) staining, cholera toxin beta (CTB) tracing, gamma synuclein staining, and terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) staining 2 weeks post operation. Flash visual evoked potentials (FVEP) and pupillary light reflex (PLR) were then tested to evaluate the function of optic nerve. The malondialdehyde (MDA) level in retina was evaluated. Results: H&E, gamma synuclein staining and CTB tracing showed that the survival rate of RGCs in hydrogen saline-treated group was significantly higher than that in saline-treated group. Apoptosis of RGCs assessed by TUNEL staining were less observed in hydrogen saline-treated group. The MDA level in retina of Hâ‚‚ group was much lower than that in placebo group. Furthermore, animals treated with hydrogen saline showed better function of optic nerve in assessments of FVEP and PLR. Conclusion: These results demonstrated that Hâ‚‚ protects RGCs and helps preserve the visual function after ONC and had a neuroprotective effect in a rat model subjected to ONC.