Hydrogen-Rich Medium Regulates Cr(VI)-Induced ER Stress and Autophagy Signaling in DF-1 Cells

Changxi Qi, Guodong Cheng, Hongyan Li, Jianzhu Liu, Kangping Liu, Xin Gao, Yongxia Liu, Yukun Cui, Zhuanglong Zhang

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DOI: 10.1007/s12011-021-02850-8 DOI is the universal ID for this study.

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Related studies have shown that chromium (Cr) is toxic to cells, and hydrogen can protect cells by regulating endoplasmic reticulum (ER) stress and autophagy. However, there are few reports on the protective effects of hydrogen on heavy metal-induced cell damage. The objective of this study was to investigate the protection of hydrogen-rich medium (HRM) on Cr(VI)-induced ER stress and autophagy in DF-1 cells. Therefore, HRM were pretreated for 30 min before Cr(VI) treatment, and detected the autophagy and ER stress-related indicators to determine the role of HRM. The results showed that HRM could reduce the cell damage caused by Cr(VI), and 3-methyladenine (3-MA) could protect cells by inhibiting over autophagy. HRM can reverse the changes of ER stress- and autophagy-related indexes caused by Cr(VI), and inhibit the excessive autophagy caused by Cr(VI). In conclusion, HRM can protect cells from damage induced by Cr(VI), and play a role by inhibiting ER stress-mediated autophagy.

Publish Year 2021
Country China
Rank Positive
Journal Biological Trace Element Research
Primary Topic Whole Body
Secondary TopicEnvironmental Pollution
Model Cell Culture
Tertiary TopicMetal Toxicity (Chromium)
Vehicle Medium (Dissolved)
pH Neutral
Application Culture Media