Hydrogen Gas Protects Kidneys from Sleep Apnea-Like Oxygen Loss

Authors
Journal
Life Sciences
Year
DOI
10.1016/j.lfs.2019.04.005
Study Type
Rat
Outcome
Positive
Peer Reviewed
Yes
Country
China
Health Condition
Chronic Intermittent Hypoxia
Body System
Renal

TL;DR

Breathing in hydrogen gas helps protect rat kidneys from damage caused by intermittent lack of oxygen.

Key Finding

Hydrogen gas inhalation protected rat kidneys from damage caused by chronic intermittent hypoxia by activating cellular cleanup mechanisms and reducing oxidative stress and cell death.

Summary

Researchers exposed rats to repeated cycles of low oxygen (a condition that mimics sleep apnea) and found that inhaling hydrogen gas protected their kidneys from damage. The hydrogen appeared to work by reducing harmful molecules called free radicals, turning on a cellular cleanup process called autophagy, and blocking stress signals that would normally trigger cell death.

Practical Takeaway

This rat study suggests hydrogen gas may have protective effects on kidney function during conditions involving repeated oxygen deprivation, such as sleep apnea. However, this is early-stage animal research, and it remains unclear whether these results would apply to humans or what dose and delivery method would be relevant. Human studies would be needed before any therapeutic recommendations could be made.

Abstract

Aims Hydrogen gas (H2) has a diversity of effects such as anti-apoptotic, anti-inflammatory and anti-oxidative properties. However, molecular mechanism underlying the potential effect of H2 on chronic intermittent hypoxia (CIH) induced renal injury remains obscure. Materials and methods In the present study, adult male Sprague-Dawley rats were randomly allocated into four groups: control (CON) group, CIH group, CIH with H2 treatment (CIH + H2) group, and control with H2 treatment (CON + H2) group. Oxidative stress, autophagy and endoplasmic reticulum (ER) stress were detected to determine how H2 affected the renal function of CIH exposed rats. Key findings We demonstrated that rats who inhale hydrogen gas showed improved renal function, alleviated pathological damage, oxidative stress and apoptosis in CIH rats. Meanwhile, CIH-induced endoplasmic reticulum stress was decreased by H2 as the expressions of CHOP, caspase-12, and GRP78 were down-regulated. Furthermore, relative higher levels of LC3-II/I ratio and Beclin-1, with decreased expression of p62, were found after H2 administrated. Inhibition of mTOR may be involved in the upregulation of autophagy by H2. Finally, increased phosphorylation of p38 and JNK was involved in the CIH-induced pathological process. H2 could inhibit the activation of p38 and JNK, suggesting H2 played an active part in resisting renal injury via MAPK. Significance Taken together, our study reveals that H2 can ameliorate CIH-induced kidney injury by decreasing endoplasmic reticulum stress and activating autophagy through inhibiting oxidative stress-dependent p38 and JNK MAPK activation.