Hydrogen gas ameliorates oxidative stress in early brain injury after subarachnoid hemorrhage in rats

Chunhua Chen, Hidenori Suzuki, John H. Zhang, Qin Hu, Xinggang Zhi, Yan Zhan

Read more:

DOI: 10.1097/CCM.0b013e31823da96d DOI is the universal ID for this study.

This link will take you to the full study.


Hydrogen gas has been demonstrated to neutralize free radicals and reduce oxidative stress recently. Our objective was to determine the therapeutic effect of H2 inhalation and its antioxidative activity on early brain injury after subarachnoid hemorrhage. Controlled in vivo laboratory study. Animal research laboratory. One hundred thirty-seven adult male Sprague-Dawley rats weighing 280-350 g. Subarachnoid hemorrhage was induced by endovascular perforation method in rats. Subarachnoid hemorrhage rats were treated with 2.9% hydrogen gas inhaled for 2 hrs after perforation. At 24 and 72 hrs, mortality, body weight, neurologic deficits, and brain water content were assessed. Blood-brain barrier permeability and apoptosis were also measured at 24 hrs. To investigate the antioxidative activity of hydrogen gas, the expression of malondialdehyde, nitrotyrosine, and 8-hydroxyguanosine, which are oxidative markers of lipid, protein, and DNA damage, respectively, were measured at 24 hrs. Hydrogen gas significantly alleviated brain edema and blood-brain barrier disruption, reduced apoptosis, and improved neurologic function at 24 hrs but not 72 hrs after subarachnoid hemorrhage. These effects were associated with the amelioration of oxidative injury of lipid, protein, and DNA. Hydrogen gas could exert its neuroprotective effect against early brain injury after subarachnoid hemorrhage by its antioxidative activity.

Publish Year 2012
Country China
Rank Positive
Journal Critical Care Medicine
Primary Topic Brain
Secondary TopicBrain Injury
Model Rat
Tertiary TopicIntracranial Hemorrhage
Vehicle Gas
pH N/A
Application Inhalation