All Studies

Simultaneous oral and inhalational intake of molecular hydrogen additively suppresses signaling pathways in rodents

Molecular hydrogen (H2) is an agent with potential applications in oxidative stress-related and/or inflammatory disorders. H2 is usually administered by inhaling H2-containing air (HCA) or by oral intake of H2-rich water (HRW). Despite mounting evidence, the molecular mechanism underlying…

Hydrogen-Rich Saline Attenuates Acute Kidney Injury After Liver Transplantation via Activating p53-Mediated Autophagy

Background: Acute kidney injury (AKI) impacts the survival of liver transplant recipients severely. To date, the related mechanism and effective therapy have not been rigorously explored. The present study aimed to explore the role of p53-mediated autophagy in the protective effect of hydrogen-rich…

Transient generation of hydrogen peroxide is responsible for carcinostatic effects of hydrogen combined with platinum nanocolloid, together with increases intracellular ROS, DNA cleavages, and proportion of G2/M-phase

In our previous study, we demonstrated that combined treatment with hydrogen (H2) and platinum nanocolloid (Pt-nc) exerted markedly antiproliferative effects on cancer cells compared with each treatment alone. However, because the related mechanisms remain unclear, we investigated carcinostatic…

Molecular hydrogen protects mice against polymicrobial sepsis by ameliorating endothelial dysfunction via an Nrf2/HO-1 signaling pathway

Endothelial injury is a primary cause of sepsis and sepsis-induced organ damage. Heme oxygenase-1 (HO-1) plays an essential role in endothelial cellular defenses against inflammation by activating nuclear factor E2-related factor-2 (Nrf2). We found that molecular hydrogen (H2) exerts…

Changes of immuno-inflammatory responses by intraperitoneal injection of hydrogen after diffuse axonal injury in rats

To explore the role of immuno-inflammatory reaction in diffuse axonal injury (DAI) and the therapeutic action of hydrogen in DAI model. Methods Totally 96 adult male SD rats were divided into 8 groups (n=12 in each): normal group, sham group, DAI groups (6h, 1d and 3d) and DAI with hydrogen…

Hydrogen-rich saline injection into the subarachnoid cavity within 2 weeks promotes recovery after acute spinal cord injury

Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis. Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. We hypothesized that hydrogen-rich saline would promote the repair of spinal cord injury induced by Allen's method…

Molecular hydrogen attenuates hypoxia/reoxygenation injury of intrahepatic cholangiocytes by activating Nrf2 expression

Hypoxia/reoxygenation (H/R) injury of cholangiocytes causes serious biliary complications during hepatobiliary surgeries. Molecular hydrogen (H2) has been shown to be effective in protecting various cells and organs against oxidative stress injury. Human liver cholangiocytes were used to determine…

Protective effects of hydrogen-rich medium on lipopolysaccharide-induced monocytic adhesion and vascular endothelial permeability through regulation of vascular endothelial cadherin

We observed the effect of hydrogen-rich medium on lipopolysaccharide (LPS)-induced human umbilical vein endothelial cells (HUVECs), hyaline leukocyte conglutination, and permeability of the endothelium. Endotheliocytes were inoculated on 6-well plates and randomly divided into 4 groups: control…

Consumption of hydrogen water prevents the occurrence of neuropathic pain in mice

Electrolytically generated acid functional water inhibits NF-<b>κ</b>B activity by attenuating nuclear-cytoplasmic shuttling of p65 and p50 subunits

Background: Human β-defensin 2 (hBD2) gene expression is dependent on nuclear factor kappa B (NF-κB) activity. We have previously demonstrated that electrolytically generated acid functional water (FW) induces the expression of hBD2 in the human oral squamous cell carcinoma (OSCC) cell line Ca9-22…

Effects of hydrogen-rich saline on early acute kidney injury in severely burned rats by suppressing oxidative stress induced apoptosis and inflammation

Early acute kidney injury (AKI) in severely burned patients predicts a high mortality that is multi-factorial. Hydrogen has been reported to alleviate organ injury via selective quenching of reactive oxygen species. This study investigated the potential protective effects of hydrogen against severe…

Administration of Hydrogen-Rich Saline in Mice with Allogeneic Hematopoietic Stem-Cell Transplantation

Background: Hydrogen, as a novel antioxidant, has been shown to selectively reduce the level of hydroxyl radicals and alleviate acute oxidative stress in many animal experiments. Hydrogen-rich saline provides a high concentration of hydrogen that can be easily and safely applied. Allogeneic…

Synergistic effect of hyperbaric oxygen preconditioning and hydrogen-rich saline in ameliorating rat flap ischemia/reperfusion injury

Aim: This study was conducted to evaluate the synergistic effects of hyperbaric oxygen (HBO) preconditioning and hydrogen-rich saline (HRS) treatment on skin flap survival and apoptosis in a rat ischemia/reperfusion (IR) skin flap model. Methods: Male Sprague-Dawley rats were randomly divided…

Effects of hydrogen inhalation on serum pro-inflammatory factors and intestinal injury in mice with severe sepsis

To investigate the effects and mechanisms of hydrogen inhalation on serum levels of pro-inflammatory factors and intestinal injury in severe septic mice. 176 male ICR mice were randomly divided into four groups: sham operation group, hydrogen control group ( sham + hydrogen inhalation ), model…

Beneficial Effects of Hydrogen-Rich Saline on Early Burn-Wound Progression in Rats

Introduction: Deep burn wounds undergo a dynamic process known as wound progression that results in a deepening and extension of the initial burn area. The zone of stasis is more likely to develop more severe during wound progression in the presence of hypoperfusion. Hydrogen has been reported…

The effects of hydrogen gas inhalation during<i>ex vivo</i>lung perfusion on donor lungs obtained after cardiac death

Lung transplantation is a well-established treatment of end-stage lung disease; however, it is limited by a shortage of donor lungs. To overcome this problem, donation after cardiac death (DCD) and ex vivo lung perfusion (EVLP) are being widely investigated. In this study, the effect of hydrogen…

Selective Antioxidative Effect of Hydrogen

Selective antioxidation is the mechanism underlying the biological effect of hydrogen, which has been widely recognized. We cannot fully understand this mechanism until we are familiar with radicals, reactive oxygen species, and oxidative damage. In order to let the readers have a general…

Three hydrogen-rich solutions protect against intestinal injury in uncontrolled hemorrhagic shock

Intestinal tissue got largely decreased blood supply in uncontrolled hemorrhagic shock, because of limited blood mainly supporting brain, heart, kidney etc. This makes intestine as the primary injury target after uncontrolled hemorrhagic shock. However, limited studies focus on how to protect…

Maternal molecular hydrogen treatment attenuates lipopolysaccharide-induced rat fetal lung injury

Maternal inflammation is associated with spontaneous preterm birth and respiratory impairment among premature infants. Recently, molecular hydrogen (H2) has been reported to have a suppressive effect on oxidative stress and inflammation. The aim of this study was to evaluate the effects of H2…

Hydrogen-rich water protects against ischemic brain injury in rats by regulating calcium buffering proteins

Hydrogen-rich water (HRW) has anti-oxidant activities, and it exerts neuroprotective effects during ischemia-reperfusion brain injury. Parvalbumin and hippocalcin are two calcium buffering proteins, which are involved in neuronal differentiation, maturation and apoptosis. The aim of this study was…