All Studies

Molecular hydrogen: A novel therapy for the treatment of pulmonary hypertension

Three hydrogen-rich solutions protect against intestinal injury in uncontrolled hemorrhagic shock

Intestinal tissue got largely decreased blood supply in uncontrolled hemorrhagic shock, because of limited blood mainly supporting brain, heart, kidney etc. This makes intestine as the primary injury target after uncontrolled hemorrhagic shock. However, limited studies focus on how to protect…

Lung inflation with hydrogen during the cold ischemia phase decreases lung graft injury in rats

Hydrogen has antioxidant and anti-inflammatory effects on lung ischemia–reperfusion injury when it is inhaled by donor or/and recipient. This study examined the effects of lung inflation with 3% hydrogen during the cold ischemia phase on lung graft function in rats. The donor lung was inflated…

Hydrogen-rich saline reduces cell death through inhibition of DNA oxidative stress and overactivation of poly (ADP-ribose) polymerase-1 in retinal ischemia-reperfusion injury

Overactivation of poly (ADP-ribose) polymerase 1 (PARP-1), as a result of sustained DNA oxidation in ischemia‑reperfusion injury, triggers programmed cell necrosis and apoptosis. The present study was conducted to demonstrate whether hydrogen‑rich saline (HRS) has a neuroprotective effect…

Hydrogen gas inhibits high-mobility group box 1 release in septic mice by upregulation of heme oxygenase 1

Sepsis is a potentially fatal whole-body inflammation caused by severe infection. Hydrogen gas (H2) is effective for treating sepsis. In this study, we hypothesized that the protective function of H2 in mice with septic lung injury occurred through the activation of heme oxygenase 1 (HO-1)…

Hydrogen-rich saline attenuates steroid-associated femoral head necrosis through inhibition of oxidative stress in a rabbit model

A growing body of evidence suggests that hydrogen is a novel, selective antioxidant that exerts a protective effect against organ damage. The present study investigated the effect of hydrogen-rich saline on corticosteroid‑induced necrosis of the femoral head in an animal model established using…

Hydrogen-rich saline may be an effective and specific novel treatment for osteoradionecrosis of the jaw

Hydrogen, a therapeutic medical gas, can exert antioxidant activity via selectively reducing cytotoxic reactive oxygen species such as hydroxyl radicals. Hydrogen-rich saline is an alternative form of molecular hydrogen that has been widely used in many studies, including metabolic syndrome…

Molecular hydrogen in sports medicine: new therapeutic perspectives

In the past 2 decades, molecular hydrogen emerged as a novel therapeutic agent, with antioxidant, anti-inflammatory and anti-apoptotic effects demonstrated in plethora of animal disease models and human studies. Beneficial effects of molecular hydrogen in clinical environment are observed…

Cigarette smoke exposure impairs reverse cholesterol transport which can be minimized by treatment of hydrogen-saturated saline

Background Cigarette smoke (CS) exposure impaired plasma lipid profiles by modification of apolipoproteins. Hydrogen (H2) has been proved effective on reducing oxidative stress or improving HDL functionalities in animal models or metabolic syndrome volunteers. This study was undertaken to explore…

Eumelanin-driven production of molecular hydrogen: A novel element of skin defense?

Molecular hydrogen (H2, dihydrogen) has been recognized as a unique cell protectant. Dihydrogen protects tissues against oxidative injuries by selectively reducing reactive oxygen species (ROS). It seems that melanin, natural pigment of skin and hair, might produce endogenous hydrogen…

Detection Techniques for Hydrogen

It is important to observe the pharmacokinetics in vivo for hydrogen bioresearch. Measuring the concentration of hydrogen is one of the key points in this study. Several methods have been developed to detect the concentration, including the gas chromatography technique, the rheophore detection…

Hydrogen Element and Hydrogen Gas

Features of hydrogen, discovery history of hydrogen, and physical and chemical properties, especially those correlated with the biological effects of hydrogen, are important for the understanding of mechanisms underlying the biological effects of hydrogen. In this chapter, we introduce…

Therapeutic Effects of Hydrogen on Different Diseases

Since 2007, hydrogen gas biology has become hotspot in medical research because of its selective antioxidant effects reported by Ohsawa et al. By the end of 2014, more than 400 papers have been published in related area, including organ ischemia-reperfusion injury, diabetes, atherosclerosis…

Future Directions in Hydrogen Studies

Although the biological effect of hydrogen was first reported in 1975, it did not draw much attention. Since the protective effects of small amount of hydrogen on oxidative stress were revealed in 2007, recent years have witnessed a tremendous development of hydrogen biology. Hydrogen has been…

Molecular hydrogen: An inert gas turns clinically effective

Molecular hydrogen (H2) appeared as an experimental agent in biomedicine approximately 40 years ago, yet the past 5 years seem to confirm its medicinal value in the clinical environment. H2 improves clinical end-points and surrogate markers in several clinical trials, from metabolic diseases…

Effects of vitamin C, vitamin E, and molecular hydrogen on the placental function in trophoblast cells

Aim: This study aimed to investigate the effects of three different antioxidants, namely vitamin C, vitamin E, and molecular hydrogen, on cytotrophoblasts in vitro. Methods: Two trophoblast cell lines, JAR and JEG-3, were exposed to different concentrations of vitamin C (0, 25, 50, 100, 500, 1,000…

Inhalation of Hydrogen Gas Is Beneficial for Preventing Contrast-Induced Acute Kidney Injury in Rats

Background: The present study aimed at investigating the effect of a novel antioxidant, hydrogen (H2) gas, on the severity of contrast-induced acute kidney injury (CIAKI) in a rat model. Methods: CIAKI was induced in rats by intravenous injection of a contrast medium, Ioversol, in addition…

Hydrogen ameliorates pulmonary hypertension in rats by anti-inflammatory and anti-oxidant effects

Objective: The pathogenesis of pulmonary arterial hypertension (PAH) involves reactive oxygen species and inflammation. Beneficial effects of molecular hydrogen, which exerts both anti-inflammatory and antioxidative effects, have been reported for various pathologic conditions. We therefore…

Hydrogen-rich saline attenuates skin ischemia/reperfusion induced apoptosis via regulating Bax/Bcl-2 ratio and ASK-1/JNK pathway

Many pathways have been reported involving the effect of hydrogen-rich saline on protecting skin flap partial necrosis induced by the inflammation of ischemia/reperfusion injury. This study focused on the influence of hydrogen-rich saline treatment on apoptosis pathway of ASK-1/JNK and Bcl-2/Bax…

Dissociative Electron Attachment to Resveratrol as a Likely Pathway for Generation of the H ₂ Antioxidant Species Inside Mitochondria

The electron-attaching properties of polyphenolic compound resveratrol were studied in vacuo by means of dissociative electron attachment (DEA) spectroscopy and in silico using density functional theory calculations. The most intense fragments generated by DEA to isolated resveratrol at thermal…