All Studies

Anti-diabetes effect of water containing hydrogen molecule and Pt nanoparticles

Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus

Electrolyzed reduced water, which is capable of scavenging reactive oxygen species, is attracting recent attention because it has shown improved efficacy against several types of diseases including diabetes mellitus. Alloxan produces reactive oxygen species and causes type 1 diabetes mellitus…

Effects of Electrolyzed Acidic Water on Infected Wounds in Mice

Acidic water with a lower pH has strong anti-bacterial effects. We investigated the effects of irrigation with electrolyzed acidic water (EAW) on infected wounds in mice. Five-week-old female C57 BL/6J mice were used; a patch of their back skin was removed and covered by a plastic sheet, that had…

Cleansing effect of the alkaline ionized water on microorganisms of the denture surface

Purpose: The purpose of this study was to evaluate the cleansing effect of recently developed alkaline ionized water, e-WASH, on microorganisms of the denture surface. Materials and methods: Removable partial and complete dentures were randomly assigned to the experimental group of 41 dentures…

<i>In vitro</i> bactericidal activity against periodontopathic bacteria by electrolyzed ion-reduced water

As typical periodontopathic bacteria, Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) were exposed to electrolyzed ion-reduced water (ERI) and ERI containing 1% sodium carboxymethylcellulose (CMC-Na) (ERI-1% CMC-Na), and the time course…

Effects of hydrogen gas inhalation on serum high mobility group box 1 levels in severe septic mice

To investigate the effect of hydrogen gas inhalation on survival rate and serum high mobility group box 1 (HMGB1) levels in severe septic mice. Severe sepsis was induced by cecal ligation and puncture (CLP) operation in mice.A total of 248 mice were randomly divided into four groups: sham operation…

Protective effect of saturated hydrogen saline against cerebral ischemia-reperfusion injury in rats

Objective To study the protective effect of saturated hydrogen saline against cerebral ischemia-reperfusion injury and the related mechanism. Methods Rat middle cerebral artery occlusion (MCAO) models were established by thread ligation of the middle cerebral artery. The rats were sacrificed 24 h…

Hydrogen therapy may be an effective and specific novel treatment for acute radiation syndrome

Hydrogen is the most abundant chemical element in the universe, however, it is seldom regarded as a therapeutic gas. Recent studies show that inhaled hydrogen gas (H(2)) has antioxidant and antiapoptotic activities that protect the brain against ischemia-reperfusion injury and stroke by selectively…

Amelioration of rat cardiac cold ischemia/reperfusion injury with inhaled hydrogen or carbon monoxide, or both

Suppressive Effect of ERW on Lipid Peroxidation and Plasma Triglyceride Level

Electrolyzed reduced water (ERW) containing hydrogen molecules and Pt nanoparticles is expected as a new antioxidant. Thiobarbituric acid reactive substances (TBARS) assay revealed that ERW had an inhibitory effect on the oxidation of low-density lipoproteins (LDL). In addition, ERW significantly…

Saturated hydrogen saline protects the lung against oxygen toxicity

Exposure to high oxygen concentrations leads to acute lung injury, including lung tissue and alveolar edema formation, congestion, intra-alveolar hemorrhage, as well as endothelial and epithelial cell apoptosis or necrosis. Several studies have reported that molecular hydrogen is an efficient…

Recent Advances in Hydrogen Research as a Therapeutic Medical Gas

Recent basic and clinical research has revealed that hydrogen is an important physiological regulatory factor with antioxidant, anti-inflammatory and anti-apoptotic protective effects on cells and organs. Therapeutic hydrogen has been applied by different delivery methods including straightforward…

Antibacterial effect of electrolysed acid water on the nasal discharge from patients with chronic rhinosinusitis

This study was conducted to determine whether electrolysed acid water (EAW) increased the antibacterial effect of irrigating solution used in the management of chronic rhinosinusitis (CRS). One hundred CRS patients were recruited from April 2008 to February 2009. Four swab specimens were taken…

Antitumor effects of nano-bubble hydrogen-dissolved water are enhanced by coexistent platinum colloid and the combined hyperthermia with apoptosis-like cell death

In order to erase reactive oxygen species (ROS) related with the proliferation of tumor cells by reducing activity of hydrogen, we developed functional water containing nano-bubbles (diameters: <900 nm for 71%/population) hydrogen of 1.1-1.5 ppm (the theoretical maximum: 1.6 ppm) with a reducing…

Suppressive effect of electrolyzed reduced water on the paraben-induced DNA damage in human dermal fibroblast cells

Parabens have been widely used as preservatives in cosmetics due to the presumed low toxicity and long history of safe use. However, recent studies have shown the potent toxicity of parabens. In order to know if electrolyzed reduced water could suppress the oxidative DNA damage of HDF cell…

Electrolysed-reduced water dialysate improves T-cell damage in end-stage renal disease patients with chronic haemodialysis

Background: T-cell damage by increased oxidative stress in end-stage renal disease (ESRD) patients undergoing chronic haemodialysis (HD) led to the increased T-cell apoptosis and the alteration of surface markers and Th1/Th2 ratio in CD4(+) T lymphocytes. Antioxidant electrolysed-reduced water…

Amelioration of rat cardiac cold ischemia/reperfusion injury with inhaled hydrogen or carbon monoxide, or both

Recent advances in novel medical gases, including hydrogen and carbon monoxide (CO), have demonstrated significant opportunities for therapeutic use. This study was designed to evaluate the effects of inhaled hydrogen or CO, or both, on cold ischemia/reperfusion (I/R) injury of the myocardium…

Hydrogen Gas Inhalation Attenuates Ventilator-Induced Lung Injury In Mice

Inhaled Hydrogen Gas Therapy for Prevention of Lung Transplant-Induced Ischemia/Reperfusion Injury in Rats

Successful abrogation of ischemia/reperfusion (I/R) injury of lung grafts could significantly improve short- and long-term outcomes for lung transplant (LTx) recipients. Hydrogen gas has potent antioxidant and antiapoptotic properties and has been recently used in number of experimental…

Intake of water with high levels of dissolved hydrogen (H2) suppresses ischemia-induced cardio-renal injury in Dahl salt-sensitive rats

Hydrogen (H(2)) reportedly produces an antioxidative effect by quenching cytotoxic oxygen radicals. We studied the biological effects of water with dissolved H(2) on ischemia-induced cardio-renal injury in a rat model of chronic kidney disease (CKD). Dahl salt-sensitive rats (7 weeks old) were…