UPLC/MS-Based Metabolomics Investigation of the Protective Effect of Hydrogen Gas Inhalation on Mice with Calcium Oxalate-Induced Renal Injury

Hongtao Lu, Jiarong Ding, Wei Chen, Wenrui Liu, Xuejun Sun, Zhiyong Guo, Zhongjiang Peng

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DOI: 10.1248/bpb.b18-00307 DOI is the universal ID for this study.

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Hydrogen has a significant protective effect on calcium oxalate-induced renal injury, but its effect on metabolic profiles is unknown. This study showed the effects of hydrogen on serum and urine metabolites in a renal injury model. Ultra-HPLC quadrupole time-of-flight-MS-based metabolomics was used to characterise metabolic variations. Twenty-five serum metabolites and 14 urine metabolites showed differences in the the nitrogen and oxygen inhalation (NO), nitrogen and oxygen inhalation combined with calcium oxalate induction (CaOx), and hydrogen inhalation combined with calcium oxalate induction (HO+CaOx) groups. Nineteen serum metabolites and 7 urine metabolites showed significant restoration to normal levels after hydrogen gas (H2) treatment. These metabolites are primarily related to amino acid metabolism, fatty acid metabolism, and phospholipid metabolism. This study showed that a comprehensive metabolomics approach is an effective strategy to elucidate the mechanisms underlying the effects of hydrogen treatment on calcium oxalate-induced renal injury.

Publish Year 2018
Country China
Rank Positive
Journal Biological and Pharmaceutical Bulletin
Primary Topic Kidney
Secondary TopicKidney Stones
Model Mouse
Tertiary TopicOxalate Injury
Vehicle Gas
pH N/A
Application Inhalation