Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus

Hanxu Yan, Jun Ye, Kiichiro Teruya, Masumi Abe, Munenori Kawamura, Noboru Nakamichi, Sanetaka Shirahata, Shigeru Kabayama, Taichi Kashiwagi, Takaaki Komatsu, Takeki Hamasaki, Tomoya Kinjo, Yuping Li

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DOI: 10.1007/s10616-010-9317-6 DOI is the universal ID for this study.

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Electrolyzed reduced water, which is capable of scavenging reactive oxygen species, is attracting recent attention because it has shown improved efficacy against several types of diseases including diabetes mellitus. Alloxan produces reactive oxygen species and causes type 1 diabetes mellitus in experimental animals by irreversible oxidative damage to insulin-producing β-cells. Here, we showed that electrolyzed reduced water prevented alloxan-induced DNA fragmentation and the production of cells in sub-G1 phase in HIT-T15 pancreatic β-cells. Blood glucose levels in alloxan-induced type 1 diabetes model mice were also significantly suppressed by feeding the mice with electrolyzed reduced water. These results suggest that electrolyzed reduced water can prevent apoptosis of pancreatic β-cells and the development of symptoms in type 1 diabetes model mice by alleviating the alloxan-derived generation of reactive oxygen species.

Publish Year 2011
Country Japan
Rank Positive
Journal Cytotechnology
Primary Topic Pancreas
Secondary TopicDiabetes (Type I)
Model Cell Culture
Tertiary TopicOxidative Stress
Vehicle Water (Electrolysis)
pH Alkaline
Application Culture Media