Protective effects of hydrogen gas against sepsis-induced acute lung injury via regulation of mitochondrial function and dynamics

Aili Dong, Can Li, Hongguang Chen, Ke-Liang Xie, Peng Zhang, Yang Yu, Yanyan Wang, Yingxue Bian, Yonghao Yu, Yungang Zhao

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DOI: 10.1016/j.intimp.2018.10.012 DOI is the universal ID for this study.

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Background: Lungs are one of the most common target organs of sepsis [1]. Hydrogen gas (H2), which has selective anti-oxidative effects, can be effectively used to treat septic mice. Mitochondrial dysfunction and dynamics play important roles in sepsis-induced organ damage.

Methods: By using cecal ligation and puncture (CLP), a classic septic model, we explored the role of 2% H2 treatment in sepsis-induced acute lung injury (ALI) linked to mitochondrial function and dynamics. We randomized male Institute for Cancer Research (ICR) mice into 4 groups: sham, sham + H2, CLP and CLP + H2. At 24 h after CLP or sham operations, we used histological examination and transmission electron microscopy (TEM) to observe lung slices. We analyzed oxygenation index (PaO2/FiO2), mitochondrial-membrane potential (MMP), adenosine triphosphate (ATP) levels, respiration control ratio (RCR) and mitochondrial-respiration complex activities (I and II) using commercial kits, and dynamin-related protein 1 (Drp1) and mitofusin-2 (MFN2) using Western blot.

Results: Therapy with 2% H2 increased PaO2/FiO2 ratios, MMP and ATP levels, RCR, complex I activity and MFN2 expression but decreased histological score and Drp1 levels in the presence of sepsis. These data indicated that inhalation of 2% H2 to regulate mitochondrial function and dynamics may be a promising therapeutic strategy for lung injuries induced by severe sepsis.

Publish Year 2018
Country China
Rank Positive
Journal International Immunopharmacology
Primary Topic Lung
Secondary TopicSepsis
Model Mouse
Tertiary TopicLung Injury
Vehicle Gas
pH N/A
Application Inhalation