Protective Effect of Hydrogen-Rich Saline on Spinal Cord Damage in Rats

Duried Alwazeer, Levent Aras, Levent Sahin, Murat Kayabas, Mustafa Makav, Serdar Yigit, Tyler W. LeBaron

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DOI: 10.3390/ph16040527 DOI is the universal ID for this study.

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The anti-inflammatory and anti-apoptotic effects of molecular hydrogen, delivered as hydrogen-rich saline (HRS), on spinal cord injury was investigated. Four-month-old male Sprague Dawley rats (n = 24) were classified into four groups: (1) control-laminectomy only at T7-T10; (2) spinal injury-dura left intact, Tator and Rivlin clip compression model applied to the spinal cord for 1 min, no treatment given; (3) HRS group-applied intraperitoneally (i.p.) for seven days; and (4) spinal injury-HRS administered i.p. for seven days after laminectomy at T7-T10 level, leaving the dura intact and applying the Tator and Rivlin clip compression model to the spinal cord for 1 min. Levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were measured in blood taken at day seven from all groups, and hematoxylin-eosin (H & E) and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) were used to stain the tissue samples. IL-6 and TNF-α levels were significantly lower in the group treated with HRS following the spinal cord injury compared to the group whose spinal cord was damaged. A decrease in apoptosis was also observed. The anti-inflammatory and anti-apoptotic effect of IL-6 may be a clinically useful adjuvant therapy after spinal cord injury.

Publish Year 2023
Country Turkey
Rank Positive
Journal Pharmaceuticals
Primary Topic Spine
Secondary TopicSpinal Cord Injury
Model Rat
Tertiary TopicOxidative Stress
Vehicle Saline (Dissolved)
pH Neutral
Application Injection