Preventive Effects of Drinking Hydrogen-Rich Water on Gingival Oxidative Stress and Alveolar Bone Resorption in Rats Fed a High-Fat Diet

Daisuke Ekuni, Hisataka Miyai, Koichiro Irie, Kouhei Fujimori, Manabu Morita, Muneyoshi Kunimoto, Takaaki Tomofuji, Tatsuji Azuma, Tatsuya Machida, Toshiki Yoneda

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DOI: 10.3390/nu9010064 DOI is the universal ID for this study.

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Obesity induces gingival oxidative stress, which is involved in the progression of alveolar bone resorption. The antioxidant effect of hydrogen-rich water may attenuate gingival oxidative stress and prevent alveolar bone resorption in cases of obesity. We examined whether hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption in obese rats fed a high-fat diet. Male Fischer 344 rats (n = 18) were divided into three groups of six rats each: a control group (fed a regular diet and drinking distilled water) and two experimental groups (fed a high-fat diet and drinking distilled water or hydrogen-rich water). The level of 8-hydroxydeoxyguanosine was determined to evaluate oxidative stress. The bone mineral density of the alveolar bone was analyzed by micro-computerized tomography. Obese rats, induced by a high-fat diet, showed a higher gingival level of 8-hydroxydeoxyguanosine and a lower level of alveolar bone density compared to the control group. Drinking hydrogen-rich water suppressed body weight gain, lowered gingival level of 8-hydroxydeoxyguanosine, and reduced alveolar bone resorption in rats on a high-fat diet. The results indicate that hydrogen-rich water could suppress gingival oxidative stress and alveolar bone resorption by limiting obesity.

Publish Year 2017
Country Japan
Rank Positive
Journal Nurtients
Primary Topic Mouth
Secondary TopicObesity
Model Rat
Tertiary TopicOxidative Stress
Vehicle Water (Electrolysis)
pH Alkaline
Application Ingestion